Nitrogen control of atrazine utilization in Pseudomonas sp strain ADP

Garcia-Gonzalez, Vicente, Govantes, Fernando, Shaw, Liz J., Burns, Richard G. and Santero, Eduardo (2003) Nitrogen control of atrazine utilization in Pseudomonas sp strain ADP. Applied and Environmental Microbiology, 69 12: 6987-6993. doi:10.1128/AEM.69.12.6987-6993.2003

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Author Garcia-Gonzalez, Vicente
Govantes, Fernando
Shaw, Liz J.
Burns, Richard G.
Santero, Eduardo
Title Nitrogen control of atrazine utilization in Pseudomonas sp strain ADP
Formatted title
Nitrogen Control of Atrazine Utilization in Pseudomonas sp. Strain ADP
Journal name Applied and Environmental Microbiology   Check publisher's open access policy
ISSN 0099-2240
Publication date 2003
Sub-type Article (original research)
DOI 10.1128/AEM.69.12.6987-6993.2003
Open Access Status File (Publisher version)
Volume 69
Issue 12
Start page 6987
End page 6993
Total pages 7
Place of publication Washington D.C., USA
Publisher American Society for Microbiology
Language eng
Subject 06 Biological Sciences
10 Technology
07 Agricultural and Veterinary Sciences
Abstract Pseudomonas sp. strain ADP uses the herbicide atrazine as the sole nitrogen source. We have devised a simple atrazine degradation assay to determine the effect of other nitrogen sources on the atrazine degradation pathway. The atrazine degradation rate was greatly decreased in cells grown on nitrogen sources that support rapid growth of Pseudomonas sp. strain ADP compared to cells cultivated on growth-limiting nitrogen sources. The presence of atrazine in addition to the nitrogen sources did not stimulate degradation. High degradation rates obtained in the presence of ammonium plus the glutamine synthetase inhibitor MSX and also with an Nas(-) mutant derivative grown on nitrate suggest that nitrogen regulation operates by sensing intracellular levels of some key nitrogen-containing metabolite. Nitrate amendment in soil microcosms resulted in decreased atrazine mineralization by the wild-type strain but not by the Nas- mutant. This suggests that, although nitrogen repression of the atrazine catabolic pathway may have a strong impact on atrazine biodegradation in nitrogen-fertilized soils, the use of selected mutant variants may contribute to overcoming this limitation.
Keyword Biotechnology & Applied Microbiology
Complete Genome Sequence
Herbicide Atrazine
Bacterial Isolate
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Agriculture and Food Sciences
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Citation counts: TR Web of Science Citation Count  Cited 49 times in Thomson Reuters Web of Science Article | Citations
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Created: Fri, 25 Jan 2008, 16:04:25 EST