Photoliberating inositol-1,4,5-trisphosphate triggers ATP release that is blocked by the connexin mimetic peptide gap 26

Braet, Katleen, Vandamme, Wouter, Martin, Patricia E. M., Evans, W. Howard and Leybaert, Luc (2003) Photoliberating inositol-1,4,5-trisphosphate triggers ATP release that is blocked by the connexin mimetic peptide gap 26. Cell Calcium, 33 1: 37-48. doi:10.1016/S0143-4160(02)00180-X


Author Braet, Katleen
Vandamme, Wouter
Martin, Patricia E. M.
Evans, W. Howard
Leybaert, Luc
Title Photoliberating inositol-1,4,5-trisphosphate triggers ATP release that is blocked by the connexin mimetic peptide gap 26
Journal name Cell Calcium   Check publisher's open access policy
ISSN 0143-4160
Publication date 2003-01
Sub-type Article (original research)
DOI 10.1016/S0143-4160(02)00180-X
Volume 33
Issue 1
Start page 37
End page 48
Total pages 12
Place of publication Edinburgh
Publisher Churchill Livingstone
Language eng
Subject 0601 Biochemistry and Cell Biology
Abstract Calcium signals can be communicated between cells by the diffusion of a second messenger through gap junction channels or by the release of an extracellular purinergic messenger. We investigated the contribution of these two pathways in endothelial cell lines by photoliberating InSP3 or calcium from intracellular caged precursors, and recording either the resulting intercellular calcium wave or else the released ATP with a luciferin/luciferase assay. Photoliberating InSP3 in a single cell within a confluent culture triggered an intercellular calcium wave, which was inhibited by the gap junction blocker alpha-glycyrrhetinic acid (alpha-GA), the connexin mimetic peptide gap 26, the purinergic inhibitors suramin, PPADS and apyrase and by purinergic receptor desensitisation. InsP(3)-triggered calcium waves were able to cross 20 mum wide cell-free zones. Photoliberating InSP3 triggered ATP release that was blocked by buffering intracellular calcium with BAPTA and by applying gap 26. Gap 26, however, did not inhibit the gap junctional coupling between the cells as measured by fluorescence recovery after photobleaching. Photoliberating calcium did not trigger intercellular calcium waves or ATP release. We conclude that InSP3-triggered ATP release through connexin hemichannels contributes to the intercellular propagation of calcium signals. (C) 2003 Elsevier Science Ltd. All rights reserved.
Keyword Cell Biology
ATP release
inositol trisphosphate
calcium signalling
flash photolysis
gap junctions
hemichannels
Intercellular Calcium Waves
Transmembrane Conductance Regulator
Human Endothelial-cells
Phospholipase-c
Ca2+ Waves
Junctional Communication
Adenosine-triphosphate
Inositol-trisphosphate
Protein-kinase
Propagation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Chemistry and Molecular Biosciences
 
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