Gene order evolution and paleopolyploidy in hemiascomycete yeasts

Wong, S, Butler, G and Wolfe, KH (2002) Gene order evolution and paleopolyploidy in hemiascomycete yeasts. Proceedings of The National Academy of Sciences of The United States of America, 99 14: 9272-9277. doi:10.1073/pnas.142101099

Author Wong, S
Butler, G
Wolfe, KH
Title Gene order evolution and paleopolyploidy in hemiascomycete yeasts
Journal name Proceedings of The National Academy of Sciences of The United States of America   Check publisher's open access policy
ISSN 0027-8424
Publication date 2002
Sub-type Article (original research)
DOI 10.1073/pnas.142101099
Open Access Status Not Open Access
Volume 99
Issue 14
Start page 9272
End page 9277
Total pages 6
Place of publication Washington
Publisher Natl Acad Sciences
Language eng
Abstract The wealth of comparative genomics data from yeast species allows the molecular evolution of these eukaryotes to be studied in great detail. We used "proximity plots" to visually compare chromosomal gene order information from 14 hemiascomycetes, including the recent Genolevures survey, to Saccharomyces cerevisiae. Contrary to the original reports, we find that the Genolevures data strongly support the hypothesis that S. cerevisiae is a degenerate polyploid. Using gene order information alone, 70% of the S. cerevisiae genome can be mapped into "sister" regions that tile together with almost no overlap. This map confirms and extends the map of sister regions that we constructed previously by using duplicated genes, an independent source of information. Combining gene order and gene duplication data assigns essentially the whole genome into sister regions, the largest gap being only 36 genes long. The 16 centromere regions of S. cerevisiae form eight pairs, indicating that an ancestor with eight chromosomes underwent complete doubling; alternatives such as segmental duplications can be ruled out. Gene arrangements in Kluyveromyces lactis and four other species agree quantitatively with what would be expected if they diverged from 5. cerevisiae before its polyploidization. In contrast, Saccharomyces exiguus, Saccharomyces servazzii, and Candida glabrata show higher levels of gene adjacency conservation, and more cases of imperfect conservation, suggesting that they split from the S. cerevisiae lineage after polyploidization. This finding is confirmed by sequences around the C. glabrata TRP1 and IPP1 loci, which show that it contains sister regions derived from the same duplication event as that of S. cerevisiae.
Keyword Multidisciplinary Sciences
Saccharomyces-cerevisiae Genome
Multiple Sequence Alignment
Fungus Ashbya-gossypii
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biomedical Sciences Publications
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Citation counts: TR Web of Science Citation Count  Cited 98 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 97 times in Scopus Article | Citations
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Created: Fri, 25 Jan 2008, 15:41:15 EST