Temporal gene regulation during HIV-1 infection of human CD4(+) T cells

Corbeil, Jacques, Sheeter, Dennis, Genini, Davide, Rought, Steffney, Leoni, Lorenzo, Du, Pinyi, Ferguson, Mark, Masys, Daniel R., Welsh, John B., Fink, J. Lynn, Sasik, Roman, Huang, David, Drenkow, Jorg, Richman, Douglas D. and Gingeras, Thomas (2001) Temporal gene regulation during HIV-1 infection of human CD4(+) T cells. Genome Research, 11 7: 1198-1204. doi:10.1101/gr.180201

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Author Corbeil, Jacques
Sheeter, Dennis
Genini, Davide
Rought, Steffney
Leoni, Lorenzo
Du, Pinyi
Ferguson, Mark
Masys, Daniel R.
Welsh, John B.
Fink, J. Lynn
Sasik, Roman
Huang, David
Drenkow, Jorg
Richman, Douglas D.
Gingeras, Thomas
Title Temporal gene regulation during HIV-1 infection of human CD4(+) T cells
Journal name Genome Research   Check publisher's open access policy
ISSN 1088-9051
Publication date 2001-07
Sub-type Article (original research)
DOI 10.1101/gr.180201
Open Access Status File (Publisher version)
Volume 11
Issue 7
Start page 1198
End page 1204
Total pages 7
Place of publication Cold Spring Harbor, NY, United States
Publisher Cold Spring Harbor Lab Press
Language eng
Abstract CD4(+) T-cell depletion is a characteristic of human immunodeficiency virus type 1 (HIV-1) infection. In this study, modulation of mRNA expression of 6800 genes was monitored simultaneously at eight time points in a CD4(+) T-cell line (CEM-GFP) during HIV infection. The responses to infection included: (1) >30% decrease at 72 h after infection in overall host-cell production of monitored mRNA synthesis, with the replacement of host-cell mRNA by viral mRNA, (2) suppression of the expression of selected mitochondrial and DNA repair gene transcripts, (3) increased expression of the proapoptotic gene and its gene p53-induced product Bax, and (4) activation of caspases 2, 3, and 9. The intense HIV-1 transcription resulted in the repression of much cellular RNA expression and was associated with the induction of apoptosis of infected cells but not bystander cells. This choreographed host gene response indicated that the subversion of the cell transcriptional machinery for the purpose of HIV-1 replication is akin to genotoxic stress and represents a major factor leading to HIV-induced apoptosis.
Keyword Biochemistry & Molecular Biology
Biotechnology & Applied Microbiology
Genetics & Heredity
P53-regulated Protein Gadd45
Tumor-suppressor P53
Nuclear Factor-i
Expression Patterns
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
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Citation counts: TR Web of Science Citation Count  Cited 24 times in Thomson Reuters Web of Science Article | Citations
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Created: Fri, 25 Jan 2008, 15:25:11 EST