Liposomal delivery of antigen to human dendritic cells

Copland, MJ, Baird, MA, Rades, T, McKenzie, JL, Becker, B, Reck, F, Tyler, PC and Davies, NM (2003) Liposomal delivery of antigen to human dendritic cells. Vaccine, 21 9-10: 883-890. doi:10.1016/S0264-410X(02)00536-4


Author Copland, MJ
Baird, MA
Rades, T
McKenzie, JL
Becker, B
Reck, F
Tyler, PC
Davies, NM
Title Liposomal delivery of antigen to human dendritic cells
Journal name Vaccine   Check publisher's open access policy
ISSN 0264-110X
0264-410X
Publication date 2003-02-14
Sub-type Article (original research)
DOI 10.1016/S0264-410X(02)00536-4
Volume 21
Issue 9-10
Start page 883
End page 890
Total pages 8
Place of publication Oxford, U.K.
Publisher Elsevier
Language eng
Subject 320501 Pharmaceutical Sciences and Pharmacy
320200 Immunology
C1
730101 Infectious diseases
11 Medical and Health Sciences
1107 Immunology
Abstract This study investigated whether formulation of antigen in mannosylated liposomes enhanced uptake and activation of dendritic cells (DC) and increased the ability of DC to induce primed T cell proliferation compared to formulation of antigen in unmodified liposomes or in solution. Immature human DC were generated from peripheral blood monocytes cultured with GM-CSF and IL-4. Uptake of antigen by DC and the degree of expression of the cell surface markers MHC class II, CD80, CD86 and the DC maturation marker CD83, was investigated by flow cytometry following incubation with liposomes or solution containing FITC-conjugated antigen. Exposure to liposomes containing FITC–ovalbumin resulted in enhanced expression of cell surface markers when compared to exposure to antigen in solution. Expression was highest following exposure to mannosylated liposomes. Mannosylated liposomes containing tetanus toxoid (TT) stimulated primed T cell proliferation more effectively than TT-neutral liposomes or TT-solution. This work suggests that mannosylated liposomes provide a versatile delivery vehicle for initiating enhanced immune responses to encapsulated peptide or protein vaccines.
Keyword Dendritic cells
Immunotherapy
Liposomes
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2004 Higher Education Research Data Collection
School of Pharmacy Publications
 
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Created: Fri, 21 Dec 2007, 11:26:02 EST by Laura McTaggart on behalf of School of Pharmacy