Techniques: High-throughput measurement of intracellular Ca2+ – back to basics

Monteith, Gregory R. and Bird, Gary St J. (2005) Techniques: High-throughput measurement of intracellular Ca2+ – back to basics. Trends in Pharmacological Sciences, 26 4: 218-223. doi:10.1016/j.tips.2005.02.002


Author Monteith, Gregory R.
Bird, Gary St J.
Title Techniques: High-throughput measurement of intracellular Ca2+ – back to basics
Formatted title
Techniques: High-throughput measurement of intracellular Ca2+ – back to basics
Journal name Trends in Pharmacological Sciences   Check publisher's open access policy
ISSN 0165-6147
Publication date 2005-04-01
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1016/j.tips.2005.02.002
Volume 26
Issue 4
Start page 218
End page 223
Total pages 6
Editor A. Smith
G. MacKenzie
S. Lewis
Place of publication Amsterdam
Publisher Elsevier in Association with The International Union of Pharmacology
Language eng
Subject 320500 Pharmacology and Pharmaceutical Sciences
Abstract High-throughput screening techniques continue to provide important tools to the pharmaceutical industry for the efficient identification of drug leads. However, high-throughput techniques are now being exploited to address a variety of pharmacological and cellular signaling research questions, including the regulation and role of intracellular Ca2+ in a plethora of biological systems. Although an awareness of specific assay conditions is crucial for reliable and reproducible measurements of intracellular free Ca2+ whichever system of study is used, the complex temporal nature of Ca2+ signals has posed some unique limitations for its measurement in high-throughput mode. Progress in high-throughput design has overcome many of these limitations and will complement other technical approaches to understanding the underlying regulation and role of intracellular Ca2+.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: Excellence in Research Australia (ERA) - Collection
School of Pharmacy Publications
 
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Created: Fri, 21 Dec 2007, 21:06:50 EST by Laura McTaggart on behalf of School of Pharmacy