A Conditional Deletion of the NR1 Subunit of the NMDA Receptor in Adult Spinal Cord Dorsal Horn Reduces NMDA Currents and Injury-Induced Pain

South, Samantha M., Kohno, Tatsuro, Kaspar, Brian K., Hegarty, Deborah, Vissel, Bryce, Drake, Carrie T., Ohata, Megumi, Jenab, Shirzad, Sailer, Andreas W., Malkmus, Shelle, Masuyama, Takashi, Horner, Philip, Bogulavsky, Johanna, Gage, Fred H., Yaksh, Tony L., Woolf, Clifford J., Heinemann, Stephen F. and Inturrisi, Charles E. (2003) A Conditional Deletion of the NR1 Subunit of the NMDA Receptor in Adult Spinal Cord Dorsal Horn Reduces NMDA Currents and Injury-Induced Pain. The Journal of Neuroscience, 23 12: 5031-5040.


Author South, Samantha M.
Kohno, Tatsuro
Kaspar, Brian K.
Hegarty, Deborah
Vissel, Bryce
Drake, Carrie T.
Ohata, Megumi
Jenab, Shirzad
Sailer, Andreas W.
Malkmus, Shelle
Masuyama, Takashi
Horner, Philip
Bogulavsky, Johanna
Gage, Fred H.
Yaksh, Tony L.
Woolf, Clifford J.
Heinemann, Stephen F.
Inturrisi, Charles E.
Title A Conditional Deletion of the NR1 Subunit of the NMDA Receptor in Adult Spinal Cord Dorsal Horn Reduces NMDA Currents and Injury-Induced Pain
Journal name The Journal of Neuroscience   Check publisher's open access policy
ISSN 1529-2401
0270-6474
Publication date 2003-06-15
Sub-type Article (original research)
Volume 23
Issue 12
Start page 5031
End page 5040
Total pages 10
Editor Woolf, Clifford J.
Heinemann, S. F.
Inturrisi, C. E.
Place of publication New York
Publisher Society for Neuroscience
Language eng
Subject 320500 Pharmacology and Pharmaceutical Sciences
320502 Basic Pharmacology
Abstract To determine the importance of the NMDA receptor (NMDAR) in pain hypersensitivity after injury, the NMDAR1 (NR1) subunit was selectively deleted in the lumbar spinal cord of adult mice by the localized injection of an adenoassociated virus expressing Cre recombinase into floxed NR1 mice. NR1 subunit mRNA and dendritic protein are reduced by 80% in the area of the virus injection, and NMDA currents, but not AMPA currents, are reduced 86–88% in lamina II neurons. The spatial NR1 knock-out does not alter heat or cold paw-withdrawal latencies, mechanical threshold, or motor function. However, injury-induced pain produced by intraplantar formalin is reduced by 70%. Our results demonstrate conclusively that the postsynaptic NR1 receptor subunit in the lumbar dorsal horn of the spinal cord is required for central sensitization, the central facilitation of pain transmission produced by peripheral injury.
Keyword NMDA receptor
conditional knock-out
Cre–loxP
formalin pain
spinal cord dorsal horn
synaptic transmission
central sensitization
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Pharmacy Publications
 
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