Methanosarcina spp. drive vinyl chloride dechlorination via interspecies hydrogen transfer

Heimann, AC, Batstone, DJ and Jakobsen, R (2006) Methanosarcina spp. drive vinyl chloride dechlorination via interspecies hydrogen transfer. Applied And Environmental Microbiology, 72 4: 2942-2949. doi:10.1128/AEM.72.4.2942-2949.2006

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Author Heimann, AC
Batstone, DJ
Jakobsen, R
Title Methanosarcina spp. drive vinyl chloride dechlorination via interspecies hydrogen transfer
Journal name Applied And Environmental Microbiology   Check publisher's open access policy
ISSN 0099-2240
Publication date 2006
Sub-type Article (original research)
DOI 10.1128/AEM.72.4.2942-2949.2006
Open Access Status File (Publisher version)
Volume 72
Issue 4
Start page 2942
End page 2949
Total pages 8
Place of publication Washington
Publisher Amer Soc Microbiology
Language eng
Abstract Two highly enriched cultures containing Dehalococcoides spp. were used to study the effect of aceticlastic methanogens on reductive vinyl chloride (VC) dechlorination. In terms of aceticlastic methanogens, one culture was dominated by Methanosaeta, while the other culture was dominated by Methanosarcina, as determined by fluorescence in situ hybridization. Cultures amended with 2-bromoethanesulfonate (BES), an efficient inhibitor of methanogens, exhibited slow VC dechlorination when grown on acetate and VC. Methanogenic cultures dominated by Methanosaeta had no impact on dechlorination rates, compared to BES-amended controls. In contrast, methanogenic cultures dominated by Methanosarcina displayed up to sevenfold-higher rates of VC dechlorination than their BES-amended counterparts. Methanosarcina-dominated cultures converted a higher percentage of [2-C-14]acetate to (CO2)-C-14 when concomitant VC dechlorination took place, compared to nondechlorinating controls. Respiratory indices increased from 0.12 in nondechlorinating cultures to 0.51 in actively dechlorinating cultures. During VC dechlorination, aqueous hydrogen (H,) concentrations dropped to 0.3 to 0.5 nM. However, upon complete VC consumption, H. levels increased by a factor of 10 to 100, indicating active hydrogen production from acetate oxidation. This process was thermodynamically favorable by means of the extremely low H-2 levels during dechlorination. VC degradation in nonmethanogenic cultures was not inhibited by BES but was limited by the availability of H2 as electron donor, in cultures both with and without BES. These findings all indicate that Methanosarcina (but not Methanosaeta), while cleaving acetate to methane, simultaneously oxidizes acetate to CO2 plus H-2, driving hydrogenotrophic dehalorespiration of VC to ethene by Dehalococcoides.
Keyword Biotechnology & Applied Microbiology
Targeted Oligonucleotide Probes
Syntrophic Acetate Oxidation
In-situ Hybridization
Reductive Dechlorination
Methanogenic Conditions
Methane Production
H-2 Transfer
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Advanced Water Management Centre Publications
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Citation counts: TR Web of Science Citation Count  Cited 30 times in Thomson Reuters Web of Science Article | Citations
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Created: Wed, 17 Oct 2007, 14:40:48 EST