CoAA, a nuclear receptor coactivator protein at the interface of transcriptional coactivation and RNA splicing

Auboeuf, Didier, Dowhan, Dennis H., Li, Xiaotao, Larkin, Kimberly, Ko, Lan, Berget, Susan M. and O'Malley, Bert W. (2004) CoAA, a nuclear receptor coactivator protein at the interface of transcriptional coactivation and RNA splicing. Molecular and Cellular Biology, 24 1: 442-453. doi:10.1128/MCB.24.1.442-453.2004

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Author Auboeuf, Didier
Dowhan, Dennis H.
Li, Xiaotao
Larkin, Kimberly
Ko, Lan
Berget, Susan M.
O'Malley, Bert W.
Title CoAA, a nuclear receptor coactivator protein at the interface of transcriptional coactivation and RNA splicing
Journal name Molecular and Cellular Biology   Check publisher's open access policy
ISSN 0270-7306
Publication date 2004-01
Sub-type Article (original research)
DOI 10.1128/MCB.24.1.442-453.2004
Open Access Status File (Publisher version)
Volume 24
Issue 1
Start page 442
End page 453
Total pages 12
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Abstract We have shown that steroid hormones coordinately control gene transcriptional activity and splicing decisions in a promoter-dependent manner. Our hypothesis is that a subset of hormonally recruited coregulators involved in regulation of promoter transcriptional activity also directly participate in alternative RNA splicing decisions. To gain insight into the molecular mechanisms by which transcriptional coregulators could control splicing decisions, we focused our attention on a recently identified coactivator, CoAA. This heterogeneous nuclear ribonucleoprotein (hnRNP)-like protein interacts with the transcriptional coregulator TRBP, a protein recruited to target promoters through interactions with activated nuclear receptors. Using transcriptional and splicing reporter genes driven by different promoters, we observed that CoAA mediates transcriptional and splicing effects in a promoter-preferential manner. We compared the activity of CoAA to the activity of other hnRNP-related proteins that, like CoAA, contain two N-terminal RNA recognition motifs (RRMs) followed by a C-terminal auxiliary domain and either have or have not been implicated in transcriptional control. By swapping either CoAA RRMs or the CoAA auxiliary domain with the corresponding domains of the proteins selected, we showed that depending on the promoter, the RRMs and the auxiliary domain of CoAA are differentially engaged in transcription. This contributes to the promoter-preferential effects mediated by CoAA on RNA splicing during the course of steroid hormone action.
Keyword Biochemistry & molecular biology
Cell biology
Dna-binding protein
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes This paper examines the ability of different steroid hormone coregulators to affect RNA processing decisions. It was concluded in the study that the precise nature of the transcriptional coregulators recruited by activated steroid receptors, depending on the promoter and cellular contexts, may play a major role in regulating the nature of RNA processing events. (Impact Factor = 10.452 )

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Created: Wed, 17 Oct 2007, 12:30:47 EST