A repeated IMP-binding motif controls oskar mRNA translation and anchoring independently of Drosophila melanogaster IMP

Munro, T. P., Kwon, S., Schnapp, B. J. and St Johnston, D. (2006) A repeated IMP-binding motif controls oskar mRNA translation and anchoring independently of Drosophila melanogaster IMP. Journal of Cell Biology, 172 4: 577-588. doi:10.1083/jcb.200510044

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads
UQ112939_OA.pdf Full text (open access) application/pdf 3.73MB 0

Author Munro, T. P.
Kwon, S.
Schnapp, B. J.
St Johnston, D.
Title A repeated IMP-binding motif controls oskar mRNA translation and anchoring independently of Drosophila melanogaster IMP
Journal name Journal of Cell Biology   Check publisher's open access policy
ISSN 0021-9525
1540-8140
Publication date 2006
Sub-type Article (original research)
DOI 10.1083/jcb.200510044
Open Access Status File (Publisher version)
Volume 172
Issue 4
Start page 577
End page 588
Total pages 12
Place of publication New York, NY, United States
Publisher Rockefeller University Press
Language eng
Abstract Zip code-binding protein 1 (ZBP-1) and its Xenopus laevis homologue, Vg1 RNA and endoplasmic reticulum-associated protein (VERA)/Vg1 RNA-binding protein (RBP), bind repeated motifs in the 3' untranslated regions (UTRs) of localized mRNAs. Although these motifs are required for RNA localization, the necessity of ZBP-1/VERA remains unresolved. We address the role of ZBP-1/VERA through analysis of the Drosophila melanogaster homologue insulin growth factor II mRNA binding protein (IMP). Using systematic evolution of ligands by exponential enrichment, we identified the IMP-binding element (IBE) UUUAY, a motif that occurs 13 times in the oskar 3'UTR. IMP colocalizes with oskar mRNA at the oocyte posterior, and this depends on the IBEs. Furthermore, mutation of all, or subsets of, the IBEs prevents oskar mRNA translation and anchoring at the posterior. However, oocytes lacking IMP localize and translate oskar mRNA normally, illustrating that one cannot necessarily infer the function of an RBP from mutations in its binding sites. Thus, the translational activation of oskar mRNA must depend on the binding of another factor to the IBEs, and IMP may serve a different purpose, such as masking IBEs in RNAs where they occur by chance. Our findings establish a parallel requirement for IBEs in the regulation of localized maternal mRNAs in D. melanogaster and X. laevis.
Keyword Cell Biology
Pole Cell-formation
Xenopus-oocytes
Posterior Pole
Intracellular-localization
Embryonic Polarity
Hnrnpa/b Homolog
Distinct Roles
Vegetal Cortex
Germ Plasm
Kh Domains
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 43 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 41 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 19 Sep 2007, 17:59:16 EST