Bioinformatics analysis of the locus for enterocyte effacement provides novel insights into type-III secretion

Pallen, Mark J., Beatson, Scott A. and Bailey, Christopher M. (2005) Bioinformatics analysis of the locus for enterocyte effacement provides novel insights into type-III secretion. BMC Microbiology, 5 9.1-9.21. doi:10.1186/1471-2180-5-9


Author Pallen, Mark J.
Beatson, Scott A.
Bailey, Christopher M.
Title Bioinformatics analysis of the locus for enterocyte effacement provides novel insights into type-III secretion
Journal name BMC Microbiology   Check publisher's open access policy
ISSN 1471-2180
Publication date 2005
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1186/1471-2180-5-9
Open Access Status DOI
Volume 5
Start page 9.1
End page 9.21
Total pages 21
Place of publication London, United Kingdom
Publisher Biomed Central
Language eng
Abstract Background: Like many other pathogens, enterohaemorrhagic and enteropathogenic strains of Escherichia coli employ a type-III secretion system to translocate bacterial effector proteins into host cells, where they then disrupt a range of cellular functions. This system is encoded by the locus for enterocyte effacement. Many of the genes within this locus have been assigned names and functions through homology with the better characterised Ysc-Yop system from Yersinia spp. However, the functions and homologies of many LEE genes remain obscure. Results: We have performed a fresh bioinformatics analysis of the LEE. Using PSI-BLAST we have been able to identify several novel homologies between LEE-encoded and Ysc-Yop-associated proteins: Orf2/YscE, Orf5/YscL, rORF8/Escl, SepQ/YscQ, SepL/YopN-TyeA, CesD2/LcrR. In addition, we highlight homology between EspA and flagellin, and report many new homologues of the chaperone CesT. Conclusion: We conclude that the vast majority of LEE-encoded proteins do indeed possess homologues and that homology data can be used in combination with experimental data to make fresh functional predictions.
Keyword Microbiology
Enteropathogenic Escherichia-coli
Translocated Intimin Receptor
Multiple Sequence Alignment
Gram-negative Bacteria
Yeast 2-hybrid System
Yersinia-pestis
Needle Complex
H-ns
Salmonella-typhimurium
Pathogenicity Island
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes This document is a journal review. Online journal article.

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: Excellence in Research Australia (ERA) - Collection
School of Chemistry and Molecular Biosciences
 
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Created: Wed, 19 Sep 2007, 18:38:21 EST