Characterization of powdered epidermal vaccine delivery with multiphoton microscopy

Mulholland, William J., Kendall, Mark A. F., White, Nick and Bellhouse, Brian J. (2004) Characterization of powdered epidermal vaccine delivery with multiphoton microscopy. Physics In Medicine And Biology, 49 22: 5043-5058. doi:10.1088/0031-9155/49/22/002

Author Mulholland, William J.
Kendall, Mark A. F.
White, Nick
Bellhouse, Brian J.
Title Characterization of powdered epidermal vaccine delivery with multiphoton microscopy
Journal name Physics In Medicine And Biology   Check publisher's open access policy
ISSN 0031-9155
Publication date 2004-11
Sub-type Article (original research)
DOI 10.1088/0031-9155/49/22/002
Volume 49
Issue 22
Start page 5043
End page 5058
Total pages 16
Place of publication Bristol
Publisher Institute of Physics
Language eng
Subject 090304 Medical Devices
110799 Immunology not elsewhere classified
Abstract Multiphoton laser scanning microscopy (MPLSM) has been adapted to non-invasively characterize hand-held powdered epidermal vaccine delivery technology. A near infrared femtosecond pulsed laser, wavelength at approximately 920 nm, was used to evoke autofluorescence of endogenous fluorophores within ex vivo porcine and human skin. Consequently, sub cellular resolution three-dimensional images of stratum corneum and viable epidermal cells were acquired and utilized to observe the morphological deformation of these cells as a result of micro-particle penetration. Furthermore, the distributional pattern of micro-particles within the specific skin target volume was quantified by measuring the penetration depth as revealed by serial optical sections in the axial plane obtained with MPLSM. Additionally, endogenous fluorescence contrast images acquired at the supra-basal layer reveal cellular structures that may pertain to dendritic Langerhans cells of the epidermis. These results show that MPLSM has advantages over conventional histological approaches, since three-dimensional functional images with sub-cellular spatial resolution to depths beyond the epidermis can be acquired non-invasively. Accordingly, we propose that MPLSM is ideal for investigations of powdered epidermal vaccine delivery.
Keyword Engineering, Biomedical
Radiology, Nuclear Medicine & Medical Imaging
Skin In-vivo
Optical Coherence Tomography
Scanning Laser Microscopy
Cytotoxic T-lymphocyte
Human Dendritic Cells
Excitation Microscopy
Langerhans Cells
Confocal Microscopy
Ballistic Delivery
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Australian Institute for Bioengineering and Nanotechnology Publications
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Citation counts: TR Web of Science Citation Count  Cited 18 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 21 times in Scopus Article | Citations
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Created: Wed, 19 Sep 2007, 17:12:19 EST