A proteomic determination of cold adaptation in the Antarctic archaeon, Methanococcoides burtonii

Goodchild, A., Saunders, N. F. W., Ertan, H., Raftery, M., Guilhaus, M, Curmi, P. M. G and Cavicchioli, R. (2004) A proteomic determination of cold adaptation in the Antarctic archaeon, Methanococcoides burtonii. Molecular Microbiology, 53 1: 309-321.


Author Goodchild, A.
Saunders, N. F. W.
Ertan, H.
Raftery, M.
Guilhaus, M
Curmi, P. M. G
Cavicchioli, R.
Title A proteomic determination of cold adaptation in the Antarctic archaeon, Methanococcoides burtonii
Journal name Molecular Microbiology   Check publisher's open access policy
ISSN 0950-382X
Publication date 2004
Sub-type Article (original research)
DOI 10.1111/j.1365-2958.2004.04130.x
Volume 53
Issue 1
Start page 309
End page 321
Total pages 13
Place of publication Oxford
Publisher Blackwell Publishing Ltd
Language eng
Subject 0605 Microbiology
1108 Medical Microbiology
Abstract A global view of the biology of the cold-adapted archaeon Methanococcoides burtonii was achieved using proteomics. Proteins specific to growth at 4degreesC versus T-opt (23degreesC) were identified by mass spectrometry using the draft genome sequence of M. burtonii. mRNA levels were determined for all genes identified by proteomics, and specific enzyme assays confirmed the protein expression results. Key aspects of cold adaptation related to transcription, protein folding and metabolism, including specific roles for RNA polymerase subunit E, a response regulator and peptidyl prolyl cis/trans isomerase. Heat shock protein DnaK was expressed during growth at T-opt, indicating that growth at 'optimal' temperatures was stressful for this cold-adapted organism. Expression of trimethylamine methyltransferase involves contiguous translation of two open reading frames, which is likely to result from incorporation of pyrrolysine at an amber stop codon. Thermal regulation in M. burtonii is achieved through complex gene expression events involving gene clusters and operons, through to protein modifications.
Keyword Biochemistry & Molecular Biology
Microbiology
Methanosarcina-barkeri
Rna-polymerase
Hyperthermophilic Archaeum
Methanogenium-frigidum
Ace Lake
Protein
Methyltransferase
Transcription
Pyrrolysine
Stress
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Chemistry and Molecular Biosciences
 
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