A new chemotaxis assay shows the extreme sensitivity of axons to molecular gradients

Rosoff, W. J., Urbach, J. S., Esrick, M. A., McAllister, R. G., Richards, L. J. and Goodhill, G. J. (2004) A new chemotaxis assay shows the extreme sensitivity of axons to molecular gradients. Nature Neuroscience, 7 6: 678-682. doi:10.1038/nn1259


Author Rosoff, W. J.
Urbach, J. S.
Esrick, M. A.
McAllister, R. G.
Richards, L. J.
Goodhill, G. J.
Title A new chemotaxis assay shows the extreme sensitivity of axons to molecular gradients
Journal name Nature Neuroscience   Check publisher's open access policy
ISSN 1097-6256
Publication date 2004-01-01
Sub-type Article (original research)
DOI 10.1038/nn1259
Volume 7
Issue 6
Start page 678
End page 682
Total pages 5
Place of publication New York
Publisher Nature Publishing Group
Language eng
Abstract Axonal chemotaxis is believed to be important in wiring up the developing and regenerating nervous system, but little is known about how axons actually respond to molecular gradients. We report a new quantitative assay that allows the long-term response of axons to gradients of known and controllable shape to be examined in a three-dimensional gel. Using this assay, we show that axons may be nature's most-sensitive gradient detectors, but this sensitivity exists only within a narrow range of ligand concentrations. This assay should also be applicable to other biological processes that are controlled by molecular gradients, such as cell migration and morphogenesis.
Keyword Neurosciences
Nerve Growth-factor
Guidance Molecules
Neurite Outgrowth
Sensory Neurons
Gel Assay
Cones
Chemoattractant
Receptors
Orient
Fibrin
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Queensland Brain Institute Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 150 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 20 Sep 2007, 03:32:29 EST