Zymogen granule exocytosis is characterized by long fusion pore openings and preservation of vesicle lipid identity

Thorn, P., Fogarty, K. E. and Parker, I. (2004) Zymogen granule exocytosis is characterized by long fusion pore openings and preservation of vesicle lipid identity. Proceedings of The National Academy of Sciences of The United States of America, 101 17: 6774-6779.


Author Thorn, P.
Fogarty, K. E.
Parker, I.
Title Zymogen granule exocytosis is characterized by long fusion pore openings and preservation of vesicle lipid identity
Journal name Proceedings of The National Academy of Sciences of The United States of America    (ERA 2010 Rank A*)   Check publisher's open access policy
Publication date 2004-04-27
Sub-type Article
DOI 10.1073/pnas.0400336101
Volume number 101
Issue number 17
ISSN 0027-8424
Start page 6774
End page 6779
Total pages 6
Place of publication Washington
Publisher National Academy of Sciences
Language eng
Subject 270100 Biochemistry and Cell Biology
Abstract The dynamics of the fusion pore that forms between a secretory vesicle and the plasma membrane are important in the regulation of both exocytosis and endocytosis. Here, we describe characteristics of fusion during zymogen granule exocytosis in exocrine pancreatic acinar cells. By using fluorescence recovery after photobleaching techniques, we show that the fusion pore remains open to allow free aqueous exchange with the vesicle lumen. There is no lipid interchange between the plasma and granule membranes during this time, and at the end of its life, the intact granule shrinks in situ, probably by a gradual pinching off of membrane patches. We propose that the protracted fusion pore lifetime is adapted to permit compound exocytosis, whereby the lingering primary granule acts as a conduit through which the contents of a secondary granule can be released. The lack of lipid intermixing may then facilitate selective recycling of granule membrane and preservation of apical membrane integrity.
Keyword Multidisciplinary Sciences
Pancreatic Acinar-cells
Synaptic Vesicles
Exocrine Cells
Membrane
Microscopy
Release
Identification
Microdomains
Resolution
Secretion
Q-Index Code C1

 
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