Elucidation of the antibacterial mechanism of the Curvularia haloperoxidase system by DNA microarray profiling

Hansen, E. H., Schembri, M. A., Klemm, P., Schafer, T., Molin, S. and Gram, L. (2004) Elucidation of the antibacterial mechanism of the Curvularia haloperoxidase system by DNA microarray profiling. Applied And Environmental Microbiology, 70 3: 1749-1757. doi:10.1128/AEM.70.3.1749-1757.2004

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Author Hansen, E. H.
Schembri, M. A.
Klemm, P.
Schafer, T.
Molin, S.
Gram, L.
Title Elucidation of the antibacterial mechanism of the Curvularia haloperoxidase system by DNA microarray profiling
Journal name Applied And Environmental Microbiology   Check publisher's open access policy
ISSN 0099-2240
Publication date 2004
Sub-type Article (original research)
DOI 10.1128/AEM.70.3.1749-1757.2004
Open Access Status File (Publisher version)
Volume 70
Issue 3
Start page 1749
End page 1757
Total pages 9
Place of publication Washington
Publisher Amer Soc Microbiology
Language eng
Abstract A novel antimicrobial enzyme system, the Curvularia haloperoxidase system, was examined with the aim of elucidating its mechanism of antibacterial action. Escherichia coli strain MG1655 was stressed with sublethal concentrations of the enzyme system, causing a temporary arrest of growth. The expression of genes altered upon exposure to the Curvularia haloperoxidase system was analyzed by using DNA microarrays. Only a limited number of genes were involved in the response to the Curvularia haloperoxidase system. Among the induced genes were the ibpA and ibpB genes encoding small beat shock proteins, a gene cluster of six genes (b0301-b0306) of unknown function, and finally, cpxP, a member of the Cpx pathway. Knockout mutants were constructed with deletions in b0301-b0306, cpxP, and cpxARP, respectively. Only the mutant lacking cpxARP was significantly more sensitive to the enzyme system than was the wild type. Our results demonstrate that DNA microarray technology cannot be used as the only technique to investigate the mechanisms of action of new antimicrobial compounds. However, by combining DNA microarray analysis with the subsequent creation of knockout mutants, we were able to pinpoint one of the specific responses of E. coli-namely, the Cpx pathway, which is important for managing the stress response from the Curvularia haloperoxidase system.
Keyword Biotechnology & Applied Microbiology
Microbiology
Escherichia-coli K-12
Shock-protein Ibpb
Heat-shock
Oxidative Stress
Gene-expression
Listeria-monocytogenes
Lactoperoxidase System
Staphylococcus-aureus
2-component System
Hydrogen-peroxide
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Chemistry and Molecular Biosciences
 
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Citation counts: TR Web of Science Citation Count  Cited 11 times in Thomson Reuters Web of Science Article | Citations
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Created: Wed, 19 Sep 2007, 17:03:48 EST