A comparative study of interspecies mating of Phratora vulgatissima and P-vitellinae using behavioural tests and molecular markers

Peacock, Lori, Batley, Jacqueline, Dungait, Jennifer, Barker, Jacqueline H. A., Powers, Stephen and Karp, Angela (2004) A comparative study of interspecies mating of Phratora vulgatissima and P-vitellinae using behavioural tests and molecular markers. Entomologia Experimentalis Et Applicata, 110 3: 231-241.


Author Peacock, Lori
Batley, Jacqueline
Dungait, Jennifer
Barker, Jacqueline H. A.
Powers, Stephen
Karp, Angela
Title A comparative study of interspecies mating of Phratora vulgatissima and P-vitellinae using behavioural tests and molecular markers
Journal name Entomologia Experimentalis Et Applicata   Check publisher's open access policy
ISSN 0013-8703
Publication date 2004-03
Sub-type Article (original research)
DOI 10.1111/j.0013-8703.2004.00143.x
Volume 110
Issue 3
Start page 231
End page 241
Total pages 11
Place of publication Oxford
Publisher Blackwell Publishing Ltd.
Language eng
Subject 0608 Zoology
Abstract The leaf beetle genus Phratora L. (Coleoptera: Chrysomelidae) has been used to study the ecology of host plant chemicals in herbivore preference, and the evolution of host use in chemical defence. Phratora vulgatissima and P. vitellinae are sympatric species distributed widely across Europe. Their trophic niches are largely separate due to strong differences in their host feeding preference, but they have occasionally been recorded together, feeding on Salix burjatica'Germany' and, only in early spring, on Populus trichocarpa (Torr & A. Gray) 'Trichobel'. Using behavioural tests and recently developed species-specific microsatellite markers, the intra- and interspecific mating of both beetle species were investigated. The microsatellite markers provided evidence that interspecific mating occurred under field conditions. Interspecific mating also took place under laboratory conditions, but less frequently than mating within species. Females of both species laid fewer eggs, and fewer eggs per clutch, when isolated with an interspecific male than with a conspecific male. Female P. vulgatissima were polyandrous, as microsatellite markers showed that their larvae were the progeny of both P. vulgatissima males that had been isolated with a single female. While only 0.55% of eggs laid in interspecific pair combinations hatched, microsatellite markers provided evidence of hybridisation between beetle species; however, these larvae died within a week when reared in a Petri dish containing 'Germany' and P. trichocarpa leaves. It can therefore be inferred that reproductive isolation is complete. The results are discussed in relation to species integrity and the implications for diverse mixtures of short-rotation coppice willow plantations.
Keyword Entomology
Chrysomelidae
Coleoptera
hybridisation
mating behaviour
microsatellites
Salix
short-rotation coppice
Multiple Paternity
Microsatellite Analysis
Beetles Coleoptera
Potato Beetles
Leaf-beetles
Chrysomelidae
Hybridization
Hybrid
Evolutionary
Population
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Agriculture and Food Sciences
 
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