Cell line-specific accumulation of the baculovirus non-hr origin of DNA replication in infected insect cells

Pijlman, G.n P., Vermeesch, A. M. G. and Vlak, J. M. (2003-11) Cell line-specific accumulation of the baculovirus non-hr origin of DNA replication in infected insect cells. Journal of Invertebrate Pathology, 84 3: 214-219.

Document type:	Journal Article
Collection:	Clinical Medical Virology Centre Publications

Author(s)	Pijlman, G.n P. Vermeesch, A. M. G. Vlak, J. M.
Title	Cell line-specific accumulation of the baculovirus non-hr origin of DNA replication in infected insect cells
Journal name	Journal of Invertebrate Pathology
Publication date	2003-11
Sub-type	Article
Volume number	84
Issue number	3
ISSN	0022-2011
Start page	214
End page	219
Total pages	6
Place of publication	Oxford
Publisher	Elsevier Science Inc.
Language	eng
Abstract	Successive Viral passage of Spodoptera exigua multicapsid nucleopolyhedrovirus (SeMNPV) in the S. exigua cell line Se301 leads to the rapid accumulation of the non-hr origin of DNA replication (ori) as large concatemers. Passage of SeMNPV in two other S. exigua cell lines, SeUCR1 and SeIZD2109, did not show the accumulation of such concatemers. When introduced into SeUCR1 and ScIZD2109 cells, the non-hr ori concatemers generated in Se301 cells were maintained but did not increase. This suggests that the non-hr ori confers a strong selective advantage in Se301 cells, but not or to a lesser extent in the other cell lines. The cell line-specific accumulation of non-hr ori concatemers might be due to a higher intrinsic recombination frequency in Se301 cells and may reflect tissue related differences involving some host cell factor(s). Since non-hr ori concatemers in Se301 cells were more abundant in intracellular than in extracellular viral DNA preparations, episomal replication and the requirement of a minimal DNA size for packaging into micleocapsids is hypothesized. (C) 2003 Elsevier Inc. All rights reserved.
Keyword(s)	Zoology SeMNPV cell line specificity non-hr ori concatemers Nuclear Polyhedrosis-virus Exigua Multicapsid Nucleopolyhedrovirus Autographa-californica Spodoptera-exigua Functional-analysis Defective Genomes Deletion Mutants Serial Passage In-vivo Identification