FimX, a multidomain protein connecting environmental signals to twitching motility in Pseudomonas aeruginosa

Huang, Bixing, Whitchurch, Cynthia B. and Mattick, John S. (2003) FimX, a multidomain protein connecting environmental signals to twitching motility in Pseudomonas aeruginosa. Journal of Bacteriology, 185 24: 7068-7076. doi:10.1128/JB.185.24.7068-7076.2003

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads
UQ111174_OA.pdf Full text (open access) application/pdf 722.77KB 0

Author Huang, Bixing
Whitchurch, Cynthia B.
Mattick, John S.
Title FimX, a multidomain protein connecting environmental signals to twitching motility in Pseudomonas aeruginosa
Formatted title
FimX, a Multidomain Protein Connecting Environmental Signals to Twitching Motility in Pseudomonas aeruginosa
Journal name Journal of Bacteriology   Check publisher's open access policy
ISSN 0021-9193
1098-5530
Publication date 2003
Sub-type Article (original research)
DOI 10.1128/JB.185.24.7068-7076.2003
Open Access Status File (Publisher version)
Volume 185
Issue 24
Start page 7068
End page 7076
Total pages 9
Editor Graham C. Walker
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2003
Language eng
Abstract Twitching motility is a form of surface translocation mediated by the extension, tethering, and retraction of type IV pili. Three independent Tn5-B21 mutations of Pseudomonas aeruginosa with reduced twitching motility were identified in a new locus which encodes a predicted protein of unknown function annotated PA4959 in the P. aeruginosa genome sequence. Complementation of these mutants with the wild-type PA4959 gene, which we designated fimX, restored normal twitching motility. fimX mutants were found to express normal levels of pilin and remained sensitive to pilus-specific bacteriophages, but they exhibited very low levels of surface pili, suggesting that normal pilus function was impaired. The fimX gene product has a molecular weight of 76,000 and contains four predicted domains that are commonly found in signal transduction proteins: a putative response regulator (CheY-like) domain, a PAS-PAC domain (commonly involved in environmental sensing), and DUF1 (or GGDEF) and DUF2 (or EAL) domains, which are thought to be involved in cyclic di-GMP metabolism. Red fluorescent protein fusion experiments showed that FimX is located at one pole of the cell via sequences adjacent to its CheY-like domain. Twitching motility in fimX mutants was found to respond relatively normally to a range of environmental factors but could not be stimulated by tryptone and mucin. These data suggest that fimX is involved in the regulation of twitching motility in response to environmental cues.
Keyword Microbiology
Type-4 Fimbrial Biogenesis
Response Regulator
Caulobacter-crescentus
Biofilm Development
Virulence Factors
Histidine Kinase
Genome Sequence
Ggdef Domain
Identification
Pilus
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Institute for Molecular Bioscience - Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 99 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 106 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 19 Sep 2007, 19:06:51 EST