Identification of pif-2, a third conserved baculovirus gene required for per os infection of insects

Pijlman, Gorben P., Pruijssers, Andrea J. P. and Vlak, Just M. (2003) Identification of pif-2, a third conserved baculovirus gene required for per os infection of insects. Journal of General Virology, 84 2041-2049. doi:10.1099/vir.0.19133-0


Author Pijlman, Gorben P.
Pruijssers, Andrea J. P.
Vlak, Just M.
Title Identification of pif-2, a third conserved baculovirus gene required for per os infection of insects
Journal name Journal of General Virology   Check publisher's open access policy
ISSN 0022-1317
Publication date 2003-08
Sub-type Article (original research)
DOI 10.1099/vir.0.19133-0
Volume 84
Start page 2041
End page 2049
Total pages 9
Place of publication Reading, England
Publisher Society for General Microbiology
Language eng
Subject 0606 Physiology
0707 Veterinary Sciences
1108 Medical Microbiology
Abstract Infection of cultured insect cells with Spodoptera exigua multicapsid nucleopolyhedrovirus (SeMNPV) resulted in the generation of mutants with major genomic deletions. Some of the mutants lacked the ability to infect S. exigua larvae per os. The gene(s) responsible for this phenotype in SeMNPV was mapped within a contiguous sequence encoding ORFs 29-35. In this paper we have shown that SeMNPV ORFs 15-35 (including genes encoding cathepsin, chitinase, GP37, PTPT-2, EGT, PKIP-1 and ARIF-1) are not essential for virus replication in cell culture or by in vivo intrahaemocoelic injection. By site-specific deletion mutagenesis of a full-length infectious clone of SeMNPV (bacmid) using ET recombination in E. coli, a series of SeMNPV bacmid mutants with increasing deletions in ORFs 15-35 was generated. Analyses of these mutants indicated that a deletion of SeMNPV ORF35 (Se35) resulted in loss of oral infectivity of polyhedral occlusion bodies. Reinsertion of ORF35 in SeMNPV bacmids lacking Se35 rescued oral infectivity. We propose the name pif-2 for Se35 and its baculovirus homologues (e.g. Autographa californica MNPV ORF22), by analogy to a different gene recently characterized in Spodoptera littoralis NPV, which was designated per os infectivity factor (pi. Similar to the p74 gene, which encodes an essential structural protein of the occlusion-derived virus envelope, pif and pif-2 belong to a group of 30 genes that are conserved among the Baculoviridae.
Keyword Biotechnology & Applied Microbiology
Virology
Nuclear Polyhedrosis-virus
Spodoptera-exigua
Genome Sequence
Deletion Mutants
Escherichia-coli
Protein-kinase
Cell-line
Nucleopolyhedrovirus
Virulence
Dna
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Chemistry and Molecular Biosciences
 
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Created: Wed, 19 Sep 2007, 17:11:34 EST