Analysis of two D1-like dopamine receptors from the honey bee Apis mellifera reveals agonist-independent activity

Mustard, J. A., Blenau, W., Hamilton, I. S., Ward, V. K., Ebert, PR and Mercer, A. R. (2003) Analysis of two D1-like dopamine receptors from the honey bee Apis mellifera reveals agonist-independent activity. Molecular Brain Research, 113 1-2: 67-77. doi:10.1016/S0169-328X(03)00091-3


Author Mustard, J. A.
Blenau, W.
Hamilton, I. S.
Ward, V. K.
Ebert, PR
Mercer, A. R.
Title Analysis of two D1-like dopamine receptors from the honey bee Apis mellifera reveals agonist-independent activity
Journal name Molecular Brain Research   Check publisher's open access policy
ISSN 0169-328X
Publication date 2003
Sub-type Article (original research)
DOI 10.1016/S0169-328X(03)00091-3
Volume 113
Issue 1-2
Start page 67
End page 77
Total pages 11
Place of publication Amsterdam
Publisher Elsevier Science Bv
Language eng
Abstract Dopamine is found in many invertebrate organisms, including insects, however, the mechanisms through which this amine operates remain unclear. We have expressed two dopamine receptors cloned from honey bee (AmDOP1 and AmDOP2) in insect cells (Spodoptera frugiperda), and compared their pharmacology directly using production of cAMP as a functional assay. In each assay, AmDOP1 receptors required lower concentrations of dopamine and 6,7-ADTN for maximal activation than AmDOP2 receptors. Conversely, butaclamol and cis(Z)-flupentixol were more potent at blocking the cAMP response mediated through AmDOP2 than AmDOP1 receptors. Expression of AmDOP1, but not AmDOP2, receptors significantly increased levels of cAMP even in the absence of ligand. This constitutive activity was blocked by cis(Z)-flupentixol. This work provides the first evidence of a constitutively activated dopamine receptor in invertebrates and suggests that although AmDOP1 and AmDOP2 share much less homology than their vertebrate counterparts, they display a number of functional parallels with the mammalian D1-like dopamine receptors. (C) 2003 Elsevier Science B.V. All rights reserved.
Keyword Neurosciences
G protein-coupled receptor
biogenic amine
invertebrate
cAMP
baculovirus
Division-of-labor
Constitutive Activity
Drosophila-melanogaster
Biogenic-amines
D1 Receptor
Mushroom Bodies
Pharmacological Characterization
Functional-characterization
Activation Properties
Inverse Agonism
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Biological Sciences Publications
 
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