Streptococcus Iniae: Serological Differences, Presence of Capsule and Resistance to Immune Serum Killing

Barnes, Andrew C., Young, Fiona M., Horne, Michael T. and Ellis, Anthony E. (2003) Streptococcus Iniae: Serological Differences, Presence of Capsule and Resistance to Immune Serum Killing. Diseases of Aquatic Organisms, 53 3: 241-247. doi:10.3354/dao053241

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Author Barnes, Andrew C.
Young, Fiona M.
Horne, Michael T.
Ellis, Anthony E.
Title Streptococcus Iniae: Serological Differences, Presence of Capsule and Resistance to Immune Serum Killing
Journal name Diseases of Aquatic Organisms   Check publisher's open access policy
ISSN 0177-5103
Publication date 2003-02
Sub-type Article (original research)
DOI 10.3354/dao053241
Open Access Status File (Publisher version)
Volume 53
Issue 3
Start page 241
End page 247
Total pages 7
Place of publication Oldendorf, Germany
Publisher Inter-Research
Language eng
Subject 0605 Microbiology
Abstract The biochemical profiles, presence of capsule, outer membrane protein profiles and serological interactions of isolates of Streptococcus iniae obtained from different geographical and fish host origins were examined. The isolates had very similar biochemical profiles using API 20 Strep but varied as to whether they were arginine dihydrolase-negative, -positive or -intermediate (AD-ve, AD+ve, AD +/- ve, respectively). Representatives of each AD type were compared in subsequent experiments. All types possessed a polysaccharide capsule. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of outer membrane proteins or whole cells revealed no difference in banding patterns between isolates. All isolates were resistant to trout normal and specific immune serum and grew well in the presence of added fresh normal serum. Serological analyses of the isolates revealed antigenic differences. Trout antiserum against the AD+ve isolate did not agglutinate the AD-ve or AD ve isolates, while antisera against the latter 2 types showed low agglutinating activity with all 3 isolates. When whole live cells of AD-ve and AD+ve isolates were dot-blotted, antiserum. to the AD+ve isolate did not stain the AD-ve isolate, but antiserum. to the AD-ve isolate stained both AD types. However, if the cells were pre-treated with Proteinase K (to remove surface-exposed protein antigens), the AD+ve isolate was stained only by its homologous antiserum. These results suggest that while certain protein antigens of the different AD type strains are immunologically cross-reactive, the capsular antigens appear to be AD type-specific. Furthermore, the results suggest that the cross-reactive antigens on the AD-ve isolate are effectively hidden by the strain-specific capsule, while they are partially exposed on the AD+ve isolate.
Keyword Fisheries
Veterinary Sciences
Streptococcus iniae
antiserum resistance
arginine dihydrolase
Trout Oncorhynchus-mykiss
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Centre for Marine Studies Publications
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Citation counts: TR Web of Science Citation Count  Cited 38 times in Thomson Reuters Web of Science Article | Citations
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Created: Wed, 19 Sep 2007, 16:41:52 EST