A comparison of antibiotic regimens in the treatment of acute melioidosis in a mouse model

Ulett, Glen C., Hirst, Robert, Bowden, Bruce, Powell, Kellie and Norton, Robert (2003) A comparison of antibiotic regimens in the treatment of acute melioidosis in a mouse model. Journal of Antimicrobial Chemotherapy, 51 1: 77-81.


Author Ulett, Glen C.
Hirst, Robert
Bowden, Bruce
Powell, Kellie
Norton, Robert
Title A comparison of antibiotic regimens in the treatment of acute melioidosis in a mouse model
Journal name Journal of Antimicrobial Chemotherapy  (ERA 2012 Listed)    (ERA 2010 Rank A)   Check publisher's open access policy
Publication date 2003
Sub-type Article
DOI 10.1093/jac/dkg011
Volume number 51
Issue number 1
ISSN 0305-7453
Start page 77
End page 81
Total pages 5
Place of publication Oxford
Publisher The British Society for Antimicrobial Chemotherapy
Language eng
Subject 1115 Pharmacology and Pharmaceutical Sciences
Abstract Melioidosis is caused by the Gram-negative bacillus Burkholderia pseudomallei. Most clinical reports of disease are from south-east Asia and northern Australia. The organism is intrinsically resistant to most commonly available antibiotics. Standard therapy includes ceftazidime either alone or in combination with co-trimoxazole. The clinical advantage in adding co-trimoxazole has never been determined; nor has the activity of newer, fourth-generation cephalosporins, such as cefepime, been studied in the treatment of this condition. BALB/c mice have been shown to represent an animal model of melioidosis. This animal model was used in this study to compare the efficacy of ceftazidime and cefepime alone or with co-trimoxazole, in the therapy of melioidosis. Antibiotic levels in the mice were determined by HPLC, and dosing was modified to keep plasma antibiotic levels at or above the MIC for the organism-antibiotic combination for a significant part of a 12 h period. Bacterial load, as determined by splenic counts, showed that ceftazidime in combination with co-trimoxazole was the most effective therapeutic option. The animal model described in this study can be used as a preliminary evaluation of therapeutic options for melioidosis.
Keyword Infectious Diseases
Microbiology
Pharmacology & Pharmacy
Burkholderia-pseudomallei Infection
C57bl/6 Mice
Balb/c
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article
Collections: Excellence in Research Australia (ERA) - Collection
School of Chemistry and Molecular Biosciences
 
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