Transcription factor complex formation and chromatin fine structure alterations at the murine c-fms (CSF-1 receptor) locus during maturation of myeloid precursor cells

Tagoh, H., Himes, R., Clarke, D., Leenen, P. J. M., Riggs, A. D., Hume, D. and Bonifer, C. (2002) Transcription factor complex formation and chromatin fine structure alterations at the murine c-fms (CSF-1 receptor) locus during maturation of myeloid precursor cells. Genes and Development, 16 13: 1721-1737. doi:10.1101/gad.222002


Author Tagoh, H.
Himes, R.
Clarke, D.
Leenen, P. J. M.
Riggs, A. D.
Hume, D.
Bonifer, C.
Title Transcription factor complex formation and chromatin fine structure alterations at the murine c-fms (CSF-1 receptor) locus during maturation of myeloid precursor cells
Journal name Genes and Development   Check publisher's open access policy
ISSN 0890-9369
1549-5477
Publication date 2002
Sub-type Article (original research)
DOI 10.1101/gad.222002
Open Access Status DOI
Volume 16
Issue 13
Start page 1721
End page 1737
Total pages 17
Place of publication Woodbury, NY, United States
Publisher Cold Spring Harbor Laboratory Press
Language eng
Abstract Expression of the gene for the macrophage colony stimulating factor receptor (CSF-1R), c-fms, has been viewed as a hallmark of the commitment of multipotent precursor cells to macrophages. Lineage-restricted expression of the gene is controlled by conserved elements in the proximal promoter and within the first intron. To investigate the developmental regulation of c-fms at the level of chromatin structure, we developed an in vitro system to examine the maturation of multipotent myeloid precursor cells into mature macrophages. The dynamics of chromatin fine structure alterations and transcription factor occupancy at the c-fms promoter and intronic enhancer was examined by in vivo DMS and UV-footprinting. We show that the c-fms gene is already transcribed at low levels in early myeloid precursors on which no CSF-1R surface expression can be detected. At this stage of myelopoiesis, the formation of transcription factor complexes on the promoter was complete. By contrast, occupancy of the enhancer was acutely regulated during macrophage differentiation. Our data show that cell-intrinsic differentiation decisions at the c-fms locus precede the appearance of c-fms on the cell surface. They also suggest that complex lineage-specific enhancers such as the c-fms intronic enhancer regulate local chromatin structure through the coordinated assembly and disassembly of distinct transcription factor complexes.
Keyword Cell Biology
Developmental Biology
Genetics & Heredity
CSF-1 receptor
chromatin
in vivo footprinting
myeloid progenitor cells
macrophage differentiation
Stimulating Factor-i
Core-binding-factor
Bone-marrow Cells
Gene-expression
Macrophage Precursors
Lysozyme Locus
Differentiation
Pu.1
Lipopolysaccharide
Activation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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Created: Wed, 19 Sep 2007, 15:27:01 EST