Amplification and detection of transposon insertion flanking sequences using fluorescent MuAFLP

Edwards, D., Coghill, J., Batley, J., Holdsworth, M. and Edwards, K. J. (2002) Amplification and detection of transposon insertion flanking sequences using fluorescent MuAFLP. Biotechniques, 32 5: 1090-1097.


Author Edwards, D.
Coghill, J.
Batley, J.
Holdsworth, M.
Edwards, K. J.
Title Amplification and detection of transposon insertion flanking sequences using fluorescent MuAFLP
Journal name Biotechniques   Check publisher's open access policy
Publication date 2002-05
Sub-type Article
Volume number 32
Issue number 5
ISSN 0736-6205
1940-9818
Start page 1090
End page 1097
Total pages 8
Place of publication New York
Publisher BioTechniques
Language eng
Abstract The amplification of transposon insertion-flanking sequences is the basis of a variety of techniques used for the detection and characterization of specific transposon insertion events. We have developed a method for the efficient size determination and quantification of amplified genomic sequences that flank Mutator (Mu) transposon insertions in maize. Using this detection method, we have been able to optimize Mu insertion site amplification and to assess amplification from increasingly complex templates representing increasing numbers of Mu-active maize plants. This detection method should be applicable for the characterization of transposon or transgene insertion events in a wide variety of organisms.
Keyword Biochemical Research Methods
Biochemistry & Molecular Biology
Dna
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article
Collections: Excellence in Research Australia (ERA) - Collection
School of Agriculture and Food Sciences
 
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Created: Wed, 19 Sep 2007, 15:18:09 EST