Assessment of Demyelination in Glycol Methacrylate Sections: a New Protocol for Cresyl Fast Violet Staining

Nguyen, K. B. and Pender, M. P. (1989) Assessment of Demyelination in Glycol Methacrylate Sections: a New Protocol for Cresyl Fast Violet Staining. Stain Technology, 64 4: 163-167.

Document type: Journal Article
Sub-type: Article
Collection: School of Medicine Publications
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Author Nguyen, K. B.
Pender, M. P.
Title Assessment of Demyelination in Glycol Methacrylate Sections: a New Protocol for Cresyl Fast Violet Staining
Journal name Stain Technology
Publication date 1989-01-01
Sub-type Article
Volume number 64
Issue number 4
ISSN 0038-9153
Start page 163
End page 167
Total pages 5
Language eng
Subject 320602 Cell Physiology
Abstract A simple staining technique for nervous tissue is described. Tissue perfused with glutaraldehyde and formaldehyde and postfixed with osmium tetroxide is embedded in glycol methacrylate. One-micrometer sections are stained with 0.05% cresyl fast violet aqueous solution at 60 C for 5 min, washed with tap water and air dried. With this method the details of all nervous tissue elements are clearly demonstrated. The technique is particularly useful for assessing demyelination because the staining of the axoplasm allows demyelinated axons to be well visualised.
Keyword Demyelination
HistoResin
High resolution light microscopy
Tissue structure
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown
Additional Notes Reprinted from Nguyen, K.B. and M.P. Pender, 'Assessment of demyelination in glycol methacrylate sections: a new protocol for cresyl fast violet staining', Stain Technology, 1989. 64(4): p. 163-7 with permission from Biological Stain Commission (http://www.biostains.org/). The journal 'Stain Technology' is now called 'Biotechnic & Histochemistry' (http://www.louisville.edu/journal/biotech-histochem/).
 
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