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Assessment of Demyelination in Glycol Methacrylate Sections: a New Protocol for Cresyl Fast Violet Staining
Nguyen, K. B. and Pender, M. P. (1989-01-01) Assessment of Demyelination in Glycol Methacrylate Sections: a New Protocol for Cresyl Fast Violet Staining. Stain Technology, 64 4: 163-167.
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| Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials) |
| Name |
Description |
MIMEType |
Size |
Downloads |
mp_st_64_4-89.pdf
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mp_st_64_4-89.pdf |
application/pdf |
576.92KB |
218 |
| Author(s) |
Nguyen, K. B.
Pender, M. P.
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| Title |
Assessment of Demyelination in Glycol Methacrylate Sections: a New Protocol for Cresyl Fast Violet Staining
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| Journal name |
Stain Technology
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| Publication date |
1989-01-01
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| Volume number |
64
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| Issue number |
4
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| Start page |
163
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| End page |
167
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| Subject |
320602 Cell Physiology
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| Abstract |
A simple staining technique for nervous tissue is described. Tissue perfused with glutaraldehyde and formaldehyde and postfixed with osmium tetroxide is embedded in glycol methacrylate. One-micrometer sections are stained with 0.05% cresyl fast violet aqueous solution at 60 C for 5 min, washed with tap water and air dried. With this method the details of all nervous tissue elements are clearly demonstrated. The technique is particularly useful for assessing demyelination because the staining of the axoplasm allows demyelinated axons to be well visualised.
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| Keyword(s) |
Demyelination
HistoResin
High resolution light microscopy
Tissue structure
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| Additional Notes |
Reprinted from Nguyen, K.B. and M.P. Pender, 'Assessment of demyelination in glycol methacrylate sections: a new protocol for cresyl fast violet staining', Stain Technology, 1989. 64(4): p. 163-7 with permission from Biological Stain Commission (http://www.biostains.org/). The journal 'Stain Technology' is now called 'Biotechnic & Histochemistry' (http://www.louisville.edu/journal/biotech-histochem/).
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