Body cavities as bioreactors to grow arteries

Campbell, Julie H., Walker, Philip J., Chue, Wai-Leng, Daly, Christopher, Cong, Hong-Liang, Xiang, Lina and Campbell, Gordon R. (2004). Body cavities as bioreactors to grow arteries. In: International Congress Series. Atherosclerosis XIII. Proceedings of the 13th International Atherosclerosis Symposium. 13th International Atherosclerosis Symposium, Tokyo, Japan, (118-121). 28 September - 2 October 2004. doi:10.1016/j.ics.2003.11.022

Author Campbell, Julie H.
Walker, Philip J.
Chue, Wai-Leng
Daly, Christopher
Cong, Hong-Liang
Xiang, Lina
Campbell, Gordon R.
Title of paper Body cavities as bioreactors to grow arteries
Conference name 13th International Atherosclerosis Symposium
Conference location Tokyo, Japan
Conference dates 28 September - 2 October 2004
Proceedings title International Congress Series. Atherosclerosis XIII. Proceedings of the 13th International Atherosclerosis Symposium
Place of Publication Amsterdam, The Netherlands
Publisher Elsevier
Publication Year 2004
Sub-type Fully published paper
DOI 10.1016/j.ics.2003.11.022
Volume 1262
Start page 118
End page 121
Total pages 4
Collection year 2004
Language eng
Formatted Abstract/Summary
‘Artificial blood vessels’ were grown in the peritoneal or pleural cavities of the dog for autologous transplantation as arterial interposition grafts. Tubing up to 250 mm long, either bare or wrapped in biodegradable polyglycolic acid (Dexon) mesh, was inserted into the body cavities using minimally invasive techniques. After 3 weeks, the tubes and their tissue capsules were harvested then the inert tubing discarded. The wall of living tissue was uniformly 1–1.5 mm thick and consisted of multiple layers of myofibroblasts that stained for α-smooth muscle actin, and extracellular matrix overlaid by a single layer of mesothelium. The bursting strength of tissue tubes with no biodegradable mesh scaffolds was in excess of 2500 mm Hg and the suture holding strength was 11.5 N. Tissue tubes (5–7 cm) were transplanted as interposition grafts into the femoral artery of the same dog in which they were grown where they remained patent until harvest at 3 to 6.5 months. Endothelial-like cells lined the lumen and the cells of wall stained for α-actin, smooth muscle myosin and smoothelin; a thick ‘neo-adventitia’ contained vasa vasorum. These studies demonstrate that peritoneal and pleural cavities of large animals can function as a bioreactor to grow tissue tubes for use as autologous vascular grafts.
©2003 Published by Elsevier B.V.

Subjects E4
321003 Cardiology (incl. Cardiovascular Diseases)
730106 Cardiovascular system and diseases
1103 Clinical Sciences
Keyword Tissue engineering
Artificial blood vessels
Q-Index Code E4

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Created: Mon, 27 Aug 2007, 13:01:27 EST