Identification of DNA markers and recombination events in the vicinity of the Fusarium Oxysporum f.sp. Lycopersici resistance gene I-3 of tomato (Lycopersicon Esculentum)

Basuki, Sesanti (2004). Identification of DNA markers and recombination events in the vicinity of the Fusarium Oxysporum f.sp. Lycopersici resistance gene I-3 of tomato (Lycopersicon Esculentum) MPhil Thesis, School of Land, Crop and Food Sciences, The University of Queensland.

       
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Author Basuki, Sesanti
Thesis Title Identification of DNA markers and recombination events in the vicinity of the Fusarium Oxysporum f.sp. Lycopersici resistance gene I-3 of tomato (Lycopersicon Esculentum)
School, Centre or Institute School of Land, Crop and Food Sciences
Institution The University of Queensland
Publication date 2004
Thesis type MPhil Thesis
Supervisor Dr Bernie Carroll
Dr. David A. Jones
Total pages 70
Collection year 2004
Language eng
Subjects L
300203 Plant Improvement (Selection, Breeding and Genetic Engineering)
620208 Vegetables
Formatted abstract

The tomato 1-3 gene originating from Lycopersicon pennellii  confers resistance against the fungal pathogen Fusarium oxysporum  f.sp. lycopersici (Fol) race 3. The gene has been mapped to chromosome 7 of tomato (Lycopersicon esculentum). As a first step toward isolation of the 1-3 gene, RAF, the randomly amplified DNA fingerprinting technique was used on near-isogenic lines (NILs) and segmental introgression lines to identify RAF markers in the vicinity of 1-3. Using this technique, approximately 7,000 RAF markers were amplified and mapped relative to 1-3 on chromosome 7. One of these RAF markers called e06 was found to cosegregate with the resistance gene analogue (RGA) 332 in a 0.28 cM window also including 1-3. This RAF marker could now be used to initiate a chromosome walk to the 1-3 locus on chromosome 7. 

RAF analysis was also employed to identify a potential novel gene for resistance to Fol race 3. The analysis revealed that an additional novel resistance gene for race 3 resides in chromosome 5 of tomato. RAF analysis proved to be a rapid and efficient technique for detecting large numbers of DNA markers linked to two genes for resistance to Fol race 3. This research should hasten plant gene isolation of the 1-3 gene on chromosome 7 by map-based cloning. The discovery of the additional gene on chromosome 5 offers the possibility of pyramiding the two genes for a more durable resistance against Fol race 3.

Keyword Tomatoes -- Genetics

Document type: Thesis
Collection: UQ Theses (RHD) - UQ staff and students only
 
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Created: Fri, 24 Aug 2007, 18:39:07 EST