Acute exposure to Cyclosporine A does not increase plasma homocysteine in rats

Austen, Simon K. (2001). Acute exposure to Cyclosporine A does not increase plasma homocysteine in rats M.Sc Thesis, School of Human Movement Studies, The University of Queensland.

       
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Author Austen, Simon K.
Thesis Title Acute exposure to Cyclosporine A does not increase plasma homocysteine in rats
School, Centre or Institute School of Human Movement Studies
Institution The University of Queensland
Publication date 2001
Thesis type M.Sc Thesis
Supervisor Dr Jeff Coombes
Dr Rob Fassett
Total pages 72
Collection year 2001
Language eng
Subjects L
321205 Nutrition and Dietetics
730215 Nutrition
Formatted abstract

Currently there is much interest in the postulate that Cyclosporine A (CyA) contributes to the hyperhomocysteinemia seen in organ transplant recipients (TxR). Hyperhomocysteinemia may, in turn, explain the increased prevalence of cardiovascular disease (CVD) seen in these patients. The purpose of this investigation was to determine the effect of CyA administration on plasma homocysteine concentration in a rat model. Twenty-four female Sprague Dawley rats (4 months old) were randomly assigned to either a treatment or control group. For eighteen days the treatment group received 25 mg/kg/d of CyA while control group rats received the same volume of the vehicle. Blood samples were taken at the mid point of the injection protocol and again following exanguination to monitor CyA concentration. At the completion of the trial, plasma was analysed for total homocysteine and renal function assessed using plasma creatinine concentrations. Whole blood was analysed for peak CyA concentration and haematological variables. Plasma homocysteine was not significantly increased compared to controls (mean ± SD: treatment = 4.79 ± 0.63 µmol/L, control = 4.46 ± 0.75 µmol/L; P=0.37) and was not correlated with final CyA concentration (r=0.17; P=0.69). There was a significant difference in renal function between the two groups [plasma creatinine (mean ± SD): treatment = 60.44 ± 7.68 µmol/L, control = 46.33 ± 1.66 µmol/L; P<0.001]. Furthermore, plasma homocysteine and renal function were positively correlated in the treatment group (r=0.73; P<0.05) but not in controls (r=0.10; P=0.81). These findings indicate that CyA decreases renal function but does not elevate plasma homocysteine in rats.  

Keyword Cyclosporine -- Physiological effect
Homocysteine -- Pathophysiology

Document type: Thesis
Collection: UQ Theses (non-RHD) - UQ staff and students only
 
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Created: Fri, 24 Aug 2007, 17:42:56 EST