The effects of ethanol on PPARS in MCF-7 human breast cancer cells

Gopisetty Venkata, N., Cabot, P. J., Monteith, G. R. and Roberts-Thomson, S. J. (2006). The effects of ethanol on PPARS in MCF-7 human breast cancer cells. In: Combined conference abstracts: ComBio 2006. ComBio 2006, Brisbane Convention Centre, Brisbane, Australia, (150-150). 24-28 September, 2006.

Author Gopisetty Venkata, N.
Cabot, P. J.
Monteith, G. R.
Roberts-Thomson, S. J.
Title of paper The effects of ethanol on PPARS in MCF-7 human breast cancer cells
Conference name ComBio 2006
Conference location Brisbane Convention Centre, Brisbane, Australia
Conference dates 24-28 September, 2006
Proceedings title Combined conference abstracts: ComBio 2006
Place of Publication Kent Town, South Australia
Publisher Australian Society for Biochemistry and Molecular Biology
Publication Year 2006
ISSN 1328-4924
Start page 150
End page 150
Total pages 1
Language eng
Abstract/Summary Peroxisome proliferator-activated receptors (PPARs) are nuclear hormone receptors involved in various metabolic diseases. In the liver, PPARα is involved in alcohol metabolism and may lead to the development of alcoholic fatty liver and other alcohol mediated liver injuries. PPARβ modulation by ethanol induces abnormal myelin production by oligodendrocytes. PPARα and PPARβ are PPAR isoforms expressed in the human breast cell lines. Epidemiological studies show a positive correlation between alcohol intake and breast cancer risk, however, the molecular mechanisms involved are unclear. We hypothesized that ethanol would affect the expression and transactivation of human PPAR isoforms in estrogen receptor (ER) positive and ER negative breast cancer cells. Using real time RT-PCR we looked at the transcription of PPAR isoforms in the presence of increasing concentrations of ethanol and saw isoform and time dependent specific effects. Gene reporter assays enabled us to ascertain the effects of ethanol on ligand-mediated activation of human PPARα and PPARβ at concentrations equivalent to both moderate and chronic alcohol consumption. Ethanol differentially blocked the ligand-mediated activation of both PPARα and PPARβ. Since PPARα and PPARβ are involved in the differentiation and proliferation of breast cancer cells, PPARs may be a possible mechanism involved in the effect of ethanol in breast cancer.
Subjects EX
320504 Toxicology (incl. Clinical Toxicology)
780105 Biological sciences
Keyword Peroxisome proliferator-activated receptors (PPARs)
Q-Index Code EX

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Created: Thu, 23 Aug 2007, 21:59:13 EST