Digital microscopy: A useful technique for measuring root elongation in solution

Blamey, Frederick P. C., Nishizawa, Naoko K. and Yoshimura, Etsuro (2005). Digital microscopy: A useful technique for measuring root elongation in solution. In: Soil Science And Plant Nutrition. 6th International Symposium on Plant-Soil Interactions at Low pH, Sendai, Japan, (705-708). 1-5 August 2004. doi:10.1111/j.1747-0765.2005.tb00096.x

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Author Blamey, Frederick P. C.
Nishizawa, Naoko K.
Yoshimura, Etsuro
Title of paper Digital microscopy: A useful technique for measuring root elongation in solution
Conference name 6th International Symposium on Plant-Soil Interactions at Low pH
Conference location Sendai, Japan
Conference dates 1-5 August 2004
Proceedings title Soil Science And Plant Nutrition   Check publisher's open access policy
Journal name Soil Science and Plant Nutrition   Check publisher's open access policy
Place of Publication Singapore, Singapore
Publisher Taylor & Francis Asia Pacific (Singapore)
Publication Year 2005
Sub-type Fully published paper
DOI 10.1111/j.1747-0765.2005.tb00096.x
Open Access Status File (Author Post-print)
ISSN 0038-0768
1747-0765
Volume 51
Issue 5
Start page 705
End page 708
Total pages 4
Language eng
Formatted Abstract/Summary
Decreased root elongation and rupture of outer cells, major effects of soluble aluminum (Al), may be studied using digital microscopy with little interference by the experimental technique. Single roots of 3-d-old mungbean (Vigna radiata L.) or soybean (Glycine max (L.) Merr.) seedlings were marked with activated charcoal particles and grown for ca. 2 h in 500 mL 1 mM CaCl2 solution at pH 6, followed by the imposition of an Al treatment. A digital image at 25-time magnification was recorded every 5 min for up to 7 h. Examination of the digital images showed that Al exerted its rhizotoxic effects rapidly (ca. 20–50 min) by reducing cell expansion in the elongation zone. Rupture of epidermal and outer cortical cells occurred later (after≥4 h) and closer to the root tip. Digital microscopy has a number of inherent benefits and problems, but is overall a valuable technique that may find wide use in studies on root growth.
Subjects 300000 Agricultural, Veterinary and Environmental Sciences
300200 Crop and Pasture Production
300203 Plant Improvement (Selection, Breeding and Genetic Engineering)
Keyword digital microscopy
Al
aluminium
root growth
mungbean
soybean
Q-Index Code E1
Q-Index Status Provisional Code
Institutional Status Non-UQ

 
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Created: Wed, 21 Jul 2004, 10:00:00 EST by Margo J Till on behalf of School of Land, Crop and Food Sciences