Phylogenomics of the Reproductive Parasite Wolbachia pipientis wMel: A Streamlined Genome Overrun by Mobile Genetic Elements

Wu, Martin, Sun, Ling V., Vamathevan, Jessica, Riegler, Markus, Deboy, Robert, Brownlie, Jeremy C., McGraw, Elizabeth A., Martin, William, Esser, Christian, Ahmadinejad, Nahal, Wiegand, Christian, Madupu, Ramana, Beanan, Maureen J., Brinkac, Lauren M., Daugherty, Sean C., Durkin, A. Scott, Kolonay, James F., Nelson, William C., Mohamoud, Yasmin, Lee, Perris, Berry, Kristi, Brook Young, M., Utterback, Teresa, Weidman, Janice, Nierman, William C., Paulsen, Ian T., Nelson, Karen E., Tettelin, Hervé, O'Neill, Scott L. and Eisen, Jonathan A. (2004) Phylogenomics of the Reproductive Parasite Wolbachia pipientis wMel: A Streamlined Genome Overrun by Mobile Genetic Elements. PLoS Biology, 2 3: 0327-0341. doi:10.1371/journal.pbio.0020069

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Author Wu, Martin
Sun, Ling V.
Vamathevan, Jessica
Riegler, Markus
Deboy, Robert
Brownlie, Jeremy C.
McGraw, Elizabeth A.
Martin, William
Esser, Christian
Ahmadinejad, Nahal
Wiegand, Christian
Madupu, Ramana
Beanan, Maureen J.
Brinkac, Lauren M.
Daugherty, Sean C.
Durkin, A. Scott
Kolonay, James F.
Nelson, William C.
Mohamoud, Yasmin
Lee, Perris
Berry, Kristi
Brook Young, M.
Utterback, Teresa
Weidman, Janice
Nierman, William C.
Paulsen, Ian T.
Nelson, Karen E.
Tettelin, Hervé
O'Neill, Scott L.
Eisen, Jonathan A.
Title Phylogenomics of the Reproductive Parasite Wolbachia pipientis wMel: A Streamlined Genome Overrun by Mobile Genetic Elements
Journal name PLoS Biology   Check publisher's open access policy
ISSN 1544-9173
1545-7885
Publication date 2004-03-01
Sub-type Article (original research)
DOI 10.1371/journal.pbio.0020069
Open Access Status DOI
Volume 2
Issue 3
Start page 0327
End page 0341
Total pages 15
Place of publication San Francisco, Calif.
Publisher Public Library of Science
Collection year 2004
Language eng
Subject 270304 Infectious Agents
270208 Molecular Evolution
270202 Genome Structure
06 Biological Sciences
Abstract The complete sequence of the 1,267,782 bp genome of Wolbachia pipientis wMel, an obligate intracellular bacteria of Drosophila melanogaster, has been determined. Wolbachia, which are found in a variety of invertebrate species, are of great interest due to their diverse interactions with different hosts, which range from many forms of reproductive parasitism to mutualistic symbioses. Analysis of the wMel genome, in particular phylogenomic comparisons with other intracellular bacteria, has revealed many insights into the biology and evolution of wMel and Wolbachia in general. For example, the wMel genome is unique among sequenced obligate intracellular species in both being highly streamlined and containing very high levels of repetitive DNA and mobile DNA elements. This observation, coupled with multiple evolutionary reconstructions, suggests that natural selection is somewhat inefficient in wMel, most likely owing to the occurrence of repeated population bottlenecks. Genome analysis predicts many metabolic differences with the closely related Rickettsia species, including the presence of intact glycolysis and purine synthesis, which may compensate for an inability to obtain ATP directly from its host, as Rickettsia can. Other discoveries include the apparent inability of wMel to synthesize lipopolysaccharide and the presence of the most genes encoding proteins with ankyrin repeat domains of any prokaryotic genome yet sequenced. Despite the ability of wMel to infect the germline of its host, we find no evidence for either recent lateral gene transfer between wMel and D. melanogaster or older transfers between Wolbachia and any host. Evolutionary analysis further supports the hypothesis that mitochondria share a common ancestor with the alpha-Proteobacteria, but shows little support for the grouping of mitochondria with species in the order Rickettsiales. With the availability of the complete genomes of both species and excellent genetic tools for the host, the wMel-D. melanogaster symbiosis is now an ideal system for studying the biology and evolution of Wolbachia infections.
Keyword parasitism
Wolbachia infections
Wolbachia pipientis
symbiosis
Q-Index Code C1
Additional Notes Originally published as:Phylogenomics of the Reproductive Parasite Wolbachia pipientis wMel: A Streamlined Genome Overrun by Mobile Genetic Elements Wu M, Sun LV, Vamathevan J, Riegler M, Deboy R, et al. The genome sequence of Wolbachia provides insights into the origins of mitochondria, as well as the ecology and evolution of endosymbiosis. Synopsis: Genome Sequence for the Intracellular Bacterium Wolbachia Primer: Endosymbiosis: Lessons in Conflict Resolution doi:10.1371/journal.pbio.0020069 Copyright: © 2004 Wu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

 
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Created: Wed, 03 Nov 2004, 10:00:00 EST