Allosteric Control Of Oxygen Binding By Haemoglobin During Embryonic Development In The Crocodile Crocodylus porosus: The Role Of Red Cell Organic Phosphates And Carbon Dioxide

Grigg, Gordon C., Wells, R. M. G. and Beard, Lynette A. (1993) Allosteric Control Of Oxygen Binding By Haemoglobin During Embryonic Development In The Crocodile Crocodylus porosus: The Role Of Red Cell Organic Phosphates And Carbon Dioxide. Journal of Experimental Biology, 175 1: 15-32.

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Author Grigg, Gordon C.
Wells, R. M. G.
Beard, Lynette A.
Title Allosteric Control Of Oxygen Binding By Haemoglobin During Embryonic Development In The Crocodile Crocodylus porosus: The Role Of Red Cell Organic Phosphates And Carbon Dioxide
Journal name Journal of Experimental Biology   Check publisher's open access policy
Publication date 1993-02-01
Sub-type Article
Volume number 175
Issue number 1
ISSN 0022-0949
Start page 15
End page 32
Total pages 18
Language eng
Subject 270105 Cellular Interactions (incl. Adhesion, Matrix, Cell Wall)
270500 Zoology
300406 Animal Growth and Development
Abstract The P50 of whole blood [30 degrees C, PCO2=2.08 kPa (15.6 mmHg)] decreases during embryonic development from approximately 6.7 kPa (50 mmHg) at 15 days to about half this value at hatching (86 days), paralleling a decrease in ATP from 100 to 5-10 micromole g-1 Hb. There is also a progressive changeover from embryonic to adult haemoglobin (HbA). A pulse of 2,3- diphosphoglycerate (2,3-DPG) (18 micromole g-1 Hb) occurs late in embryonic life. It has no effect on whole-blood oxygen-affinity and falls rapidly at hatching to values typical of post-hatchling crocodilians in general (<1.0 micromole g-1 Hb). ATP has a marked effect on the oxygen affinity of embryonic haemoglobin (HbE) but not on HbA. 2,3-DPG has only very small effects on the oxygen affinities of HbE and HbA. CO2 has a small effect on the oxygen affinity of HbE but a marked effect on that of HbA. Values of PO2 measured in the chorio-allantoic artery [2.9 kPa (22 mmHg)] and vein [5.9 kPa (52 mmHg)] imply an increase in saturation from approximately 30 % to more than 80 %. Neither whole-blood oxygen-affinity nor ATP level was altered in response to an experimental 7-day exposure to low ambient oxygen levels [10.7 kPa (80 mmHg)]. The results do not lend themselves easily to the pan-selectionist paradigm in which all physiological traits are viewed as being adaptive.
Keyword embryo development
crocodile
Crocodylus porosus
oxygen binding
haemoglobin
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown
Additional Notes Copyright 1996 The Company of Biologists.

Document type: Journal Article
Sub-type: Article
Collection: School of Biological Sciences Publications
 
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