Ovine model of scarless wound healing in the 80-day merino fetus 2

Fraser, J. F., Cuttle, L., Kempf, M., Phillips, G., Choo, K., Muller, M. and Kimble, R. (2003). Ovine model of scarless wound healing in the 80-day merino fetus 2. In: The Australian and New Zealand journal of surgery: Abstracts of the 2002 Annual Scientific Meeting of the Surgical Research Society of Australasia. 2002 Annual Scientific Meeting of the Surgical Research Society of Australasia, x, (A5-A5). 2002. doi:10.1046/j.1445-2197.2003.t01-1-02581.x


Author Fraser, J. F.
Cuttle, L.
Kempf, M.
Phillips, G.
Choo, K.
Muller, M.
Kimble, R.
Title of paper Ovine model of scarless wound healing in the 80-day merino fetus 2
Conference name 2002 Annual Scientific Meeting of the Surgical Research Society of Australasia
Conference location x
Conference dates 2002
Proceedings title The Australian and New Zealand journal of surgery: Abstracts of the 2002 Annual Scientific Meeting of the Surgical Research Society of Australasia   Check publisher's open access policy
Place of Publication Carlton, Vic.
Publisher Blackwell Publishing Asia
Publication Year 2003
Sub-type Published abstract
DOI 10.1046/j.1445-2197.2003.t01-1-02581.x
Open Access Status
ISSN 1445-1433
Volume 73
Issue 4
Start page A5
End page A5
Total pages 1
Language eng
Formatted Abstract/Summary
Introduction: It is known that the fetus heals an incisional wound in a scarless fashion. To date, there has been no study of the fetal response to deep dermal partial thickness burn injury (DDPTBI). We first created a standardized model of DDPTBI in the Merino fetus and lamb and then created this standardized injury in 21 fetal and post natal lambs concurrently, to examine the differences in the wound healing processes.
Methods:
Twenty-one Merino fetal lambs (80 days gestation) and Merino lambs (30 days) each received a standard DDPTBI, using hot water scald. The scald area was tattooed with India Ink. Animals were then serially killed at day 1,3,5,7,14,21,60. At post-mortem, sections of tissue were taken from both lamb and fetus. This tissue was blocked, and 4 ∝m sections were stored for light microscopy and immunohistochemically stained for αSMA content. We then calculated αSMA content on each slide. Results: The fetal burn could only be identified by tattoo marks after day 7. There was no visible or microscopic scarring in the fetal wounds post day 7. The wound area in the lamb was always visible, palpable and identifiable under light microscopy. There was a significant rise in αSMA content in fetal and lamb burned tissue as compared to control fetal and lamb tissue. There was highly significant differences in the αSMA content of fetal burn vs. lamb burn tissue (P < 0.01 and P < 0.005, respectively). Conclusion: The fetal lamb heals a DDPTBI in a scarless fashion, and the lamb heals a similar injury with scarring. The minimal rise in αSMA content seen in fetal tissue in response to DDPTI as compared to the much larger rise in lamb tissue with the same injury may be partly responsible for the scar-less wound healing seen in the fetus.
Subjects EX
730204 Child health
110323 Surgery
Q-Index Code EX
Q-Index Status Provisional Code

 
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Created: Thu, 23 Aug 2007, 19:09:36 EST