http://espace.library.uq.edu.au/ The University of Queensland en Fez http://blogs.law.harvard.edu/tech/rss http://espace.library.uq.edu.au/view/UQ:141058 The polymerisation kinetics of 1,1'-(methylenedi-1,4-phenylene)bismaleimide (MDP-BMI) with the aromatic diamine 1,1'-diaminodiphenylmethane (DDM) has been investigated. Fourier-transform near infrared (FT-NIR) spectroscopy was used to obtain quantitative data for the various functional groups in BMI/DDM mixtures with respect to time during isothermal cure over the temperature range 160-180 degrees C. Quantitative rate data were obtained for all the functional groups in the resins by mathematical differentiation of the time dependence of the concentrations. A mechanism for the polymerisation has been deduced from this rate data, in which the rate-determining step was found to involve a reaction between the primary or secondary amine and a hydrogen bonded complex formed between the maleimide and amine groups. The rare coefficients for the reaction of the amines with the complex were found to be four times larger for the primary amine than that for the secondary amine. The activation energy for the cure over this temperature range was found to be 43 kJ mol(-1). (C) 2000 Elsevier Science Ltd. All rights reserved. 2008-06-10T00:00:00Z Hopewell, JL og George, GA og Hill, DJT http://espace.library.uq.edu.au/view/UQ:58672 The effects of thermodynamic non-ideality on the forms of sedimentation equilibrium distributions for several isoelectric proteins have been analysed on the statistical-mechanical basis of excluded volume to obtain an estimate of the extent of protein solvation. Values of the effective solvation. parameter delta are reported for ellipsoidal as well as spherical models of the proteins, taken to be rigid, impenetrable macromolecular structures. The dependence of the effective solvated radius upon protein molecular mass exhibits reasonable agreement with the relationship calculated for a model in which the unsolvated protein molecule is surrounded by a 0.52-nm solvation shell. Although the observation that this shell thickness corresponds to a double layer of water molecules may be of questionable relevance to mechanistic interpretation of protein hydration, it augurs well for the assignment of magnitudes to the second virial coefficients of putative complexes in the quantitative characterization of protein-protein interactions under conditions where effects of thermodynamic non-ideality cannot justifiably be neglected. (C) 2001 Elsevier Science B.V. All rights reserved. 2007-08-14T00:00:00Z Winzor, D. J. og Carrington, L. E. og Harding, S. E. http://espace.library.uq.edu.au/view/UQ:70992 Pilin is the major subunit of the essential virulence factor pili and is glycosylated at Ser63. In this study we investigated the gene pglI to determine whether it is involved in the biosynthesis of the pilin-linked glycan of Neisseria meningitidis strain C311#3. A N. meningitidis C311#3pglI mutant resulted in a change of apparent molecular weight in SDS-PAGE and altered binding of antisera, consistent with a role in the biosynthesis of the pilin-linked glycan. These data, in conjunction with homology with well-characterised acyltransferases suggests a specific role for pglI in the biosynthesis of the basal 2,4-diacetamido-2,4,6-trideoxyhexose residue of the pilin-linked glycan. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Microbiological Societies. 2007-08-15T00:00:00Z Warren, Matthew J. og Roddam, Louise F. og Power, Peter M. og Terry, Tamsin D. og Jennings, Michael P. http://espace.library.uq.edu.au/view/UQ:182023 2009-09-03T09:05:57Z Sharp, E. L. og Davis-Poynter, N. J. og Farrell, H. E. http://espace.library.uq.edu.au/view/UQ:35787 Sulfonation is an important metabolic process involved in the excretion and in some cases activation of various endogenous compounds and xenobiotics. This reaction is catalyzed by a family of enzymes named sulfotransferases. The cytosolic human sulfotransferases SULT1A1 and SULT1A3 have overlapping yet distinct substrate specificities. SULT1A1 favors simple phenolic substrates such as p-nitrophenol, whereas SULT1A3 prefers monoamine substrates such as dopamine. In this study we have used a variety of phenolic substrates to functionally characterize the role of the amino acid at position 146 in SULT1A1 and SULT1A3. First, the mutation A146E in SULT1A1 yielded a SULT1A3-like protein with respect to the Michaelis constant for simple phenols. The mutation E146A in SULT1A3 resulted in a SULT1A1-like protein with respect to the Michaelis constant for both simple phenols and monoamine compounds. When comparing the specificity of SULT1A3 toward tyramine with that for p-ethylphenol (which differs from tyramine in having no amine group on the carbon side chain), we saw a 200-fold preference for tyramine. The kinetic data obtained with the E146A mutant of SULT1A3 for these two substrates clearly showed that this protein preferred substrates without an amine group attached. Second, changing the glutamic acid at position 146 of SULT1A3 to a glutamine, thereby neutralizing the negative charge at this position, resulted in a 360-fold decrease in the specificity constant for dopamine. The results provide strong evidence that residue 146 is crucial in determining the substrate specificity of both SULT1A1 and SULT1A3 and suggest that there is a direct interaction between glutamic acid 146 in SULT1A3 and monoamine substrates. 2007-08-13T00:00:00Z Brix, Lulu A. og Barnett, Amanda C. og Duggleby, Ronald G. og Leggett, Barbara og McManus, Michael E. http://espace.library.uq.edu.au/view/UQ:65073 This review summarizes the development of exclusion chromatography, also termed gel filtration, molecular-sieve chromatography and gel permeation chromatography, for the quantitative characterization of solutes and solute interactions. As well as affording a means of determining molecular mass and molecular mass distribution, the technique offers a convenient way of characterizing solute selfassociation and solute-ligand interactions in terms of reaction stoichiometry and equilibrium constant. The availability of molecular-sieve media with different selective porosities ensures that very little restriction is imposed on the size of solute amenable to study. Furthermore, access to a diverse array of assay procedures for monitoring the column eluate endows analytical exclusion chromatography with far greater flexibility than other techniques from the viewpoint of solute concentration range that can be examined. In addition to its widely recognized prowess as a means of solute separation and purification, exclusion chromatography thus also possesses considerable potential for investigating the functional roles of the purified solutes. (C) 2003 Elsevier Science B.V. All rights reserved. 2007-08-14T00:00:00Z Winzor, D. J. http://espace.library.uq.edu.au/view/UQ:72333 2007-08-14T00:00:00Z Fuerst, J A og Webb, R I og van Niftrik, L. og Jetten, M. S. og Strous, M. http://espace.library.uq.edu.au/view/UQ:282668 2012-10-02T15:20:44Z Zhou, Guangmin og Wang, Da-Wei og Hou, Peng-Xiang og Li, Wenshan og Li, Na og Liu, Chang og Li, Feng og Cheng, Hui-Ming http://espace.library.uq.edu.au/view/UQ:85195 We describe the development of a capture enzyme-linked immunosorbent assay for the detection of the dengue virus nonstructural protein NS1. The assay employs rabbit polyclonal and monoclonal antibodies as the capture and detection antibodies, respectively. Immunoaffinity-purified NS1 derived from dengue 2 virus-infected cells was used as a standard to establish a detection sensitivity of approximately 4 ng/ml for an assay employing monoclonal antibodies recognizing a dengue 2 serotype-specific epitope. A number of serotype cross-reactive monoclonal antibodies were also shown to be suitable probes for the detection of NS1 expressed by the remaining three dengue virus serotypes. Examination of clinical samples demonstrated that the assay was able to detect NS1 with minimal interference from serum components at the test dilutions routinely used, suggesting that it could form the basis of a useful additional diagnostic test for dengue virus infection. Furthermore, quantitation of NS1 levels in patient sera may prove to be a valuable surrogate marker for viremia. Surprisingly high levels of NS1, as much as 15 mu g/ml, were found in acute-phase sera taken hom some of the patients experiencing serologically confirmed dengue 2 virus secondary infections but was not detected in the convalescent sera of these patients. In contrast, NS1 could not be detected in either acute-phase or convalescent serum samples taken from patients with serologically confirmed primary infection. The presence of high levels of secreted NS1 in the sera of patients experiencing secondary dengue virus infections, and in the context of an anamnestic antibody response, suggests that NS1 may contribute significantly to the formation of the circulating immune complexes that are suspected to play an important role in the pathogenesis of severe dengue disease. 2007-08-19T00:00:00Z Young, Paul R. og Hilditch, Paige A. og Bletchly, Cheryl og Halloran, Wendy http://espace.library.uq.edu.au/view/UQ:257350 2011-10-20T09:05:22Z Su, Leo Y. og Willner, Dana L. og Segall, Anca M. http://espace.library.uq.edu.au/view/UQ:219536 2010-11-02T00:00:00Z Crossley, M. J. og Burn, P. L. http://espace.library.uq.edu.au/view/UQ:290928 A new, strongly absorbing narrow optical gap small molecule acceptor YF25 is reported. When YF25 is blended with the archetypal donor P3HT, significant photocurrent red of the polymer optical gap is produced in addition to the normal response generated by the donor polymer. Using EQE and TRMC measurements we show that the additional photocurrent red of the donor edge is derived from excitation of the electron acceptor and subsequent hole-transfer to P3HT. 2013-02-10T00:51:18Z Fang, Yuan og Pandey, Ajay K. og Nardes, Alexandre M. og Kopidakis, Nikos og Burn, Paul L. og Meredith, Paul http://espace.library.uq.edu.au/view/UQ:143316 2008-06-10T00:00:00Z Arin, M. J. og Longley, M. A. og Kuster, W. og Huber, M. og Hohl, D. og Rothnagel, J. A. og Roop, D. R. http://espace.library.uq.edu.au/view/UQ:143490 2008-06-10T00:00:00Z Moore, C. J. og Possner, S. og Hayes, P. og Paddon-Jones, G. C. og Kitching, W. http://espace.library.uq.edu.au/view/UQ:171039 2009-03-20T12:02:03Z Yang, Hua Gui og Sun, Cheng Hua og Qiao, Shi Zhang og Zou, Jin og Liu, Gang og Smith, Sean Campbell og Cheng, Hui Ming og Lu, Gao Qing http://espace.library.uq.edu.au/view/UQ:163280 nature, most bacteria live in close association with surfaces as complex communities referred to as biofilms. Community members within these compact microbial consortia show extraordinary resistance to conventional antibiotics, biocides, and hydrodynamic shear forces when compared to their planktonic counterparts. The buildup of these surface-associated bacterial communities is a highly organized and complex process that requires many signal transduction mechanisms to orchestrate the different stages of development. In this review, we describe several types of signal transduction that Gram-negative bacteria employ during the adhesion and expansion stages of biofilm formation, as well as discuss quorum-sensing in relation to the production of virulence factors. 2009-02-06T00:00:00Z Schembri, Mark A. og Givskov, Michael og Klemm, Ker http://espace.library.uq.edu.au/view/UQ:245936 2011-08-21T02:14:10Z Williams, Simon J. og Sornaraj, Pradeep og deCourcy-Ireland, Emma og Menz, R. Ian og Kobe, Bostjan og Ellis, Jeffrey G. og Dodds, Peter N. og Anderson, Peter A. http://espace.library.uq.edu.au/view/UQ:261385 The Automated force field Topology Builder (ATB, http://compbio.biosci.uq.edu.au/atb) is a Web-accessible server that can provide topologies and parameters for a wide range of molecules appropriate for use in molecular simulations, computational drug design, and X-ray refinement. The ATB has three primary functions: (1) to act as a repository for molecules that have been parametrized as part of the GROMOS family of force fields, (2) to act as a repository for pre-equilibrated systems for use as starting configurations in molecular dynamics simulations (solvent mixtures, lipid systems pre-equilibrated to adopt a specific phase, etc.), and (3) to generate force field descriptions of novel molecules compatible with the GROMOS family of force fields in a variety of formats (GROMOS, GROMACS, and CNS). Force field descriptions of novel molecules are derived using a multistep process in which results from quantum mechanical (QM) calculations are combined with a knowledge-based approach to ensure compatibility (as far as possible) with a specific parameter set of the GROMOS force field. The ATB has several unique features: (1) It requires that the user stipulate the protonation and tautomeric states of the molecule. (2) The symmetry of the molecule is analyzed to ensure that equivalent atoms are assigned identical parameters. (3) Charge groups are assigned automatically. (4) Where the assignment of a given parameter is ambiguous, a range of possible alternatives is provided. The ATB also provides several validation tools to assist the user to assess the degree to which the topology generated may be appropriate for a given task. In addition to detailing the steps involved in generating a force field topology compatible with a specific GROMOS parameter set (GROMOS 53A6), the challenges involved in the automatic generation of force field parameters for atomic simulations in general are discussed. 2011-11-14T22:19:55Z Malde, Alpeshkumar K. og Zuo, Le og Breeze, Matthew og Stroet, Martin og Poger, David og Nair, Pramod C. og Oostenbrink, Chris og Mark, Alan E. http://espace.library.uq.edu.au/view/UQ:274945 2012-05-30T10:05:11Z Peters, Kate M. og Edwards, Stacey L. og Nair, Shalima S. og French, Juliet D. og Bailey, Peter J. og Salkield, Kathryn og Stein, Sandra og Wagner, Sarah og Francis, Glenn D. og Clark, Susan J. og Brown, Melissa A. http://espace.library.uq.edu.au/view/UQ:196073 2010-02-21T00:02:41Z Stephens, Evan og Ross, Ian L. og King, Zachary og Mussgnug, Jan H. og Kruse, Olaf og Posten, Clemens og Borowitzka, Michael A. og Hankamer, Ben http://espace.library.uq.edu.au/view/UQ:79404 Attention is drawn to a need for caution in the determination of binding data for protein-polyelectrolyte interactions by frontal analysis continuous capillary electrophoresis (FACCE). Because the method is valid only for systems involving comigration of complex(es) and slower-migrating reactant, establishing conformity with that condition is clearly a prerequisite for its application. However, that requirement has not been tested in any published studies thus far. On the basis of calculated FACCE patterns, presented to illustrate features by which such comigration of complex(es) and slower-migrating reactant can be identified, the form of the published pattern for a P-lactoglobulin-poly(styrenesulfonate) mixture does not seem to signify the migration behavior required to justify its consideration in such terms. Additional experimental studies are therefore needed to ascertain the validity of FACCE as a means of determining binding data for the characterization of protein-polyelectrolyte interactions. (c) 2005 Elsevier Inc. All rights reserved. 2007-08-15T00:00:00Z Winzor, D. J. http://espace.library.uq.edu.au/view/UQ:166526 2009-03-09T00:00:00Z Cosgrove, Kelly L. og McGeary, Ross P. http://espace.library.uq.edu.au/view/UQ:8642 We consider systems in which electroactive enzymes are immobilised on an electrode surface through physical adsorption or covalent attachment on an electrode surface and substrate(s), product(s) and inhibitor(s) are present in the bulk solution. We solve the governing equations numerically by fully implicit finite differences (FIFD). Our numerical method relies on the formation of a sparse matrix from matrix blocks, which we call the kinetic block, containing kinetic terms for the enzyme reactions, and mass transport block(s) which contain the terms for the mass transport of substrate(s), product(s) and inhibitor(s). The resultant non-linear sparse matrix equation is solved using the sparse matrix solver in the MATHEMATICA kernel which in turn uses UMFPACK multifrontal direct solver methods and Krylov iterative methods preconditioned by an incomplete LU factorization. Due to the non-linear nature of the problem the solution is iterated at each time step until the desired degree of precision is obtained. Adaptation to a variety of mechanisms is performed by changing the terms in the kinetic block and the boundary conditions in the mass transport blocks. Adaptation to a number of different voltammetric methods is achieved by changing one or two lines of code describing the how applied potential changes with time. 2006-02-14T00:00:00Z Honeychurch, Michael og Bernhardt, Paul http://espace.library.uq.edu.au/view/UQ:191228 2010-01-04T13:06:06Z Kang, Seungha og Denham, Stuart E. og Morrison, Mark og Zhongtang, Yu og McSweeney, Chris S. http://espace.library.uq.edu.au/view/UQ:275139 2012-06-01T14:07:57Z Shiddiky, Muhammad J. A. og Kithva, Prakash H. og Kozak, Darby og Trau, Matt http://espace.library.uq.edu.au/view/UQ:258100 The Ixodes holocyclus tick causes paralysis in up to 10,000 companion and domestic animals each year in Australia. Treatment requires the removal of the parasite and the administration of a commercial tick antiserum that is prepared from hyperimmune dogs. Each batch of this serum is initially tested for toxin-neutralising potency in a mouse bioassay that is expensive, time consuming, and subjective. With the aim of developing a rapid in vitro assay to replace the bioassay, we used a partially purified antigen prepared from I. holocyclus salivary glands to develop an ELISA to detect toxin-reactive antibodies in hyperimmune dog sera. The optimised ELISA reliably detected antibodies reactive to I. holocyclus salivary gland antigens. Parallel testing of sera with a negative control antigen prepared from the salivary glands of the nontoxic tick Rhipicephalus (Boophilus) microplus provided further evidence that we were detecting toxin-specific antibodies in the assay. Using the ELISA, we could also detect antibodies induced in rats after experimental infestation with I. holocyclus. This assay shows promise as an alternative means of assessing the potency of batches of hyperimmune dog serum and to screen for toxin-reactive monoclonal antibodies produced from immunised rodents. 2011-10-20T00:00:00Z Hall-Mendelin, S. og O'Donoghue, P. og Atwell, R. og Lee, R. og Hall, R.A. http://espace.library.uq.edu.au/view/UQ:232225 2011-03-07T15:43:11Z Gilbert, R.G. http://espace.library.uq.edu.au/view/UQ:96688 2007-08-24T00:00:00Z Pow, D. V. og Sullivan, R. og Reye, P. og Scott, H. L. http://espace.library.uq.edu.au/view/UQ:58941 Six polyesters were synthesised from 4,4 ' -oxy-bis(benzoyl chloride) and 1,4-, 1,5-, 1,6-, 2,3-, 2,6-, and 2,7-naphthalenediol isomers. The structures of the polyesters were characterised by means of IR, inherent viscosities in tetrachloroethane (TCE), solutions at 303 K and thermal analysis. The glass transition temperatures were in the range of 425-494 K by DSC thermal analysis. All of the polyesters were irradiated in an AECL Gammacell 220 unit at a dose rate of approximately 6.7 kGy/h to doses in the range of 0-15 kGy at 77 and 300 K. ESR spectroscopy was used to examine the radicals formed during radiolysis and to measure their yields. The G-values for radical formation in the polyesters were found to be in the range 0.18-1.41 at 77 K and 0.19-0.78 at 300 K. At 77 K, up to 15% of the radicals formed on radiolysis were found to be photo-bleachable anion radicals. Annealing experiments were carried out in order to identify the neutral radicals, which were assigned to naphthyl- or phenyl- and phenoxyl-type radicals. (C) 2001 Elsevier Science Ltd. All rights reserved. 2007-08-14T00:00:00Z Hill, D. J. T. og Choi, B. K. og Ahn, H. K. og Choi, E. J. http://espace.library.uq.edu.au/view/UQ:76882 The radiolysis of a poly(ethylene-co-propylene), Elpro, marketed by Thai Polypropylene Co. Ltd for the manufacture of medical goods has been investigated at 77 K. Calcium stearate was blended with the Elpro as a processing aid; and dioctyl phthalate, DOP, was added in various amounts as a radiation stabilizer. The ESR spectra of Elpro and Elpro+Ca were very similar and characterized principally by the presence of PP a-carbon radicals. The spectra of the samples containing DOP were similar to those for Elpro but with an additional narrow singlet arising from DOP radicals. On annealing the irradiated polymers to higher temperatures, the singlet was lost between 250 and 270 K, and at room temperature the principal radicals remaining were allyl radicals. The G-values for radical formation at 77 K for Elpro and Elpro+Ca at 77 K were 3.0 and 3.2, respectively, but incorporation of DOP resulted in lower G-values, ranging from 1.6 to 1.4 for 0.5 and 2.5 phr DOP, respectively.(c) 2005 Wiley Periodicals, Inc. 2007-08-15T00:00:00Z Fuzail, M. og Hill, D. J. T. og Le, T. T. http://espace.library.uq.edu.au/view/UQ:58944 The formation of radicals in poly(vinyl alcohol), PVA, powder irradiated at 77 K by gamma -rays and the transformations of these radicals during photolysis with visible wavelengths and on thermal annealing have been studied. After irradiation a four-line ESR spectrum was observed. It was assigned to a triplet of the C-alpha-radical (38%), with a splitting of 3.27 mT, superimposed on a doublet (62%) with a splitting of 2.7 mT. The doublet appears to be composed of two radicals, one of which is photo-bleachable (58%) and the other which is not photo-bleachable (42%). This suggests that the latter radical is a neutral radical. The photo-bleachable component of the doublet has been assigned to a carbonyl anion radical. but the second doublet due to a neutral radical is unassigned. The total G-value for formation of radicals at 77 K was found to be 2.41 +/- 0.03. Upon illumination with visible light, the anion radicals were removed and the doublet components or the spectrum diminished in intensity, while the three-line spectrum of the C-alpha-radical became more clearly visible. This transition was due to the photo-detachment of electrons from traps which were proposed to be located on carbonyl groups in the polymer resulting from incomplete hydrolysis of the vinyl acetate. The photo-decay of the anion radicals could be satisfactorily described by a two-stage process. The first stage comprised the decay of approximately 80% of the anion radicals present, while the second stage was associated with the decay of the remaining 20%. Subsequent thermal annealing of a photolysed sample to 290 K led to a change in the shape of the spectrum to form a more clearly defined triplet, As the doublet of the neutral radical decays on thermal annealing between 150 and 250K, the C-alpha-radical is formed. (C) 2001 Elsevier Science Ltd. All rights reserved. 2007-08-14T00:00:00Z Zainuddin, Z. og Hill, D. J. T. og Le, T. T. http://espace.library.uq.edu.au/view/UQ:148388 2008-06-06T00:00:00Z Tavelin, S. og Hashimoto, K. og Lazarova, L. og Malkinson, J. P. og Toth, I. og Artursson, P. http://espace.library.uq.edu.au/view/UQ:70936 2007-08-15T00:00:00Z Tyagi, R. og Lai, R. Y. og Duggleby, R.G. http://espace.library.uq.edu.au/view/UQ:36916 Cardicola forsteri sp. nov. (Digenea: Sanguinicolidae) is described from the heart of captive southern blue-fin tuna, Thunnus maccoyii (Scombridae), from South Australia. The new species is distinguished from other species of Cardicola by its very extensive testis, the length of its oesophagus, the length of its gut caeca and the form of its ovary. Cardicola smithi appears to be associated with heart and gill lesions(1). 2007-08-13T00:00:00Z Cribb, T. H. og Daintith, M. og Munday, B. http://espace.library.uq.edu.au/view/UQ:36104 A previously unknown chemolithoautotrophic arsenite-oxidizing bacterium has been isolated from a gold mine in the Northern Territory of Australia. The organism, designated NT-26, was found to be a gram-negative motile rod with two subterminal flagella. In a minimal medium containing only arsenite as the electron donor (5 mM), oxygen as the electron acceptor, and carbon dioxide-bicarbonate as the carbon source, the doubling time for chemolithoautotrophic growth was 7.6 h. Arsenite oxidation was found to be catalyzed by a periplasmic arsenite oxidase (optimum pH, 5.5). Based upon 16S rDNA phylogenetic sequence analysis, NT-26 belongs to the Agrobacterium/Rhizbium branch of the alpha-Proteobacteria and may represent a new species. This recently discovered organism is the most rapidly growing chemolithoautotrophic arsenite oxidizer known. 2007-08-13T00:00:00Z Santini, J. M. og Sly, L. I. og Schnagl, R. D. og Macy, J. M. http://espace.library.uq.edu.au/view/UQ:218699 2010-10-18T00:00:00Z Barnard, Ross T. http://espace.library.uq.edu.au/view/UQ:286241 2012-11-25T00:23:02Z Tandy, Kristen og Dutta, Gitish K. og Zhang, Yuliang og Venkatramaiah, N. og Aljada, Muhsen og Burn, Paul L. og Meredith, Paul og Namdas, Ebinazar B. og Patil, Satish http://espace.library.uq.edu.au/view/UQ:219521 2010-11-02T00:00:00Z Boardman, F. H. og Grice, A. W. og Ruther, M. G. og Sheldon, T. J. og Bradley, D. D. C. og Burn, P. L. http://espace.library.uq.edu.au/view/UQ:281170 2012-09-04T17:23:48Z Glasson, Christopher R. K. og Meehan, George V. og Clegg, Jack K. og Lindoy, Leonard F. og Turner, Peter og Duriska, Martin B. og Willis, Rick http://espace.library.uq.edu.au/view/UQ:195630 2010-02-17T14:36:52Z Poger, David og van Gunsteren, Wilfred F. og Mark, Alan E. http://espace.library.uq.edu.au/view/UQ:98122 2007-08-24T00:00:00Z Warren, M. J. og Roddam, L. F. og Power, P. og Dieckelmann, M. og Jennings, M. P. http://espace.library.uq.edu.au/view/UQ:8952 Lobosorchis tibaldiae n. gen. and n. sp. (Digenea: Cryptogonimidae) is described from the intestine, pyloric ceca, and rectum of 2 species of Lutjanus (Pisces: Lutjanidae), Lutjanus carponotatus and Lutjanus fulviflamma, from the Great Barrier Reef, Queensland, Australia, and New Caledonia. The genus is tentatively placed in the Neochasminae and is distinguished within the Cryptogonimidae by the combination of follicular testes, oral spines, and vitelline follicles restricted to the anterior region of the body not extending posteriorly to the ventral sucker. 2006-06-08T00:00:00Z Miller, Terrence L. og Cribb, Thomas H. http://espace.library.uq.edu.au/view/UQ:98730 2007-08-24T00:00:00Z Nolan, M. J. og Cribb, T. H. http://espace.library.uq.edu.au/view/UQ:9472 A novel heterobinuclear mixed valence complex [Fe^IIICu^II(BPBPMP)(OAc)_2]ClO_4, 1, with the unsymmetrical N_5O_2 donor ligand 2-bis[{(2-pyridylmethyl)aminomethyl}-6-{(2-hydroxybenzyl)(2-pyridylmethyl)} aminomethyl]-4-methylphenol (H_2BPBPMP) has been synthesized and characterized. A combination of data from mass spectrometry, potentiometric titrations, X-ray absorption and electron paramagnetic resonance spectroscopy, as well as kinetics measurements indicates that in ethanol/water solutions an [Fe^III-(nu)OH-Cu^IIOH_2]+ species is generated which is the likely catalyst for 2,4-bis(dinitrophenyl)phosphate and DNA hydrolysis. Insofar as the data are consistent with the presence of an Fe_III-bound hydroxide acting as a nucleophile during catalysis, 1 presents a suitable mimic for the hydrolytic enzyme purple acid phosphatase. Notably, 1 is significantly more reactive than its isostructural homologues with different metal composition (Fe^IIIM^II, where M^II is Zn^II, Mn^II, Ni^II,or Fe^II). Of particular interest is the observation that cleavage of double-stranded plasmid DNA occurs even at very low concentrations of 1 (2.5 nuM), under physiological conditions (optimum pH of 7.0), with a rate enhancement of 2.7 x 10^7 over the uncatalyzed reaction. Thus, 1 is one of the most effective model complexes to date, mimicking the function of nucleases. 2005-07-22T00:00:00Z Lanznaster, Mauricio og Neves, Ademir og Bortoluzzi, Adailton J. og Aires, Veronika V. E. og Szpoganicz, Bruno og Terenzi, Hernan og Severino, Patricia C. og Fuller, Julie M. og Drew, Simon C. og Gahan, Lawrence R. og Hanson, Graeme R. og Riley, Mark J. og Schenk, Gerhard http://espace.library.uq.edu.au/view/UQ:296096 2013-04-07T00:15:27Z Hobson-Peters, Jody og Yam, Alice Wei Yee og Lu, Jennifer Wei Fei og Setoh, Yin Xiang og May, Fiona J. og Kurucz, Nina og Walsh, Susan og Prow, Natalie A. og Davis, Steven S. og Weir, Richard og Melville, Lorna og Hunt, Neville og Webb, Richard I. og Blitvich, Bradley J. og Whelan, Peter og Hall, Roy A. http://espace.library.uq.edu.au/view/UQ:270009 2012-03-15T00:00:00Z Khamsan, Sorachai og Liawruangrath, Saisunee og Teerawutkulrag, Aphiwat og Pyne, Stephen G. og Garson, Mary J. og Liawruangrath, Boonsom http://espace.library.uq.edu.au/view/UQ:140245 2008-06-10T00:00:00Z Bray, R. A. og Cribb, T. H. http://espace.library.uq.edu.au/view/UQ:281150 2012-09-04T17:19:35Z Wenzel, Marco og Gloe, Kerstin og Gloe, Karsten og Bernhard, Gert og Clegg, Jack K. og Ji, Xue-Kui og Lindoy, Leonard F. http://espace.library.uq.edu.au/view/UQ:232388 2011-03-07T15:49:52Z Leslie, Gregory L. og Maxwell, Ian A. og Ballard, Mathew J. og Gilbert, Robert G. og Napper, Donald H. http://espace.library.uq.edu.au/view/UQ:219539 2010-11-02T00:00:00Z Crossley, M. J. og Burn, P. L. og Langford, S. J. og Pyke, S. M. og Stark, A. G.